Biomolecular Breadboards:Protocols:cost estimate
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Cell-Free Circuit Breadboard Cost Estimate
Reaction
E. coli crude extract
"Amount per 3X prep" refers to the amount of crude extract you would make following the protocol E. Coli Crude Extract Preparation, 3X. On average, one prep yields 10.5 mL of extract, which is enough for about 31500 cell-free circuit reactions.
| Ingredient | Bulk Quantity | Bulk Price | Amount per 3X prep | Price per 3X prep | Source | Catalog Number |
|---|---|---|---|---|---|---|
| 2xYT | 1 kg | $87.3 | 139.93 g | $12.216 | MP biomedicals | 3012-032 |
| Bacto-Agar | 1 lb | $171.11 | 0.44 g | $0.17 | BD (from Fisher) | 214010 |
| Chloramphenicol powder | 5 g | $30.50 | 3.876 mg | $0.02 | Sigma-Aldrich | C1919 |
| DTT | 5 g | $97.40 | 0.927 g | $18.06 | Sigma-Aldrich | D0632 |
| Ethanol (100%) | 500 mL | $54.68 | 114 uL | $0.01 | Fisher | BP2818 |
| K-glutamate | 500 g | $161.50 | 48.8 g | $15.76 | Sigma-Aldrich | G1149 |
| Mg-glutamate | 250 g | $47.10 | 21.76 g | $4.10 | Sigma-Aldrich | 49605 |
| Potassium phosphate monobasic solution | 1 L | $43.80 | 90.15 mL | $3.95 | Sigma-Aldrich | P8709 |
| Potassium phosphate dibasic solution | 1 L | $38.00 | 163.92 mL | $6.23 | Sigma-Aldrich | P8584 |
| Tris | 1 kg | $133.58 | 13.325 g | $1.78 | Fisher | BP1521 |
| Total Price | $62.30 |
| Disposable Material | Bulk Quantity | Bulk Price | Amount per 3X prep | Price per 3X prep | Source | Catalog Number |
|---|---|---|---|---|---|---|
| 2xYT | 1 kg | $87.3 | 139.93 g | $12.216 | MP biomedicals | 3012-032 |
| Beads (0.1 mm diameter) | 1 lb | $34.00 | 80 g | $6.00 | BioSpec | 11079101 |
| Bead beating tubes | 500 count | $141.00 | 42 count | $11.84 | BioSpec | 522S |
| Bead filters | 100 count | $112.00 | 6 count | $6.72 | Bio-Rad | 732-6204 |
| Bio-rad Bradford protein assay kit (BSA) | 1 kit | $123.00 | 1 assay | $0.62 | Bio-Rad | 500-0201 |
| Cuvettes | 500 count | $170.30 | 7 count | $2.38 | Fisher | 14-955-127 |
| Dialysis cassettes | 10 count | $85.40 | 6 count | $51.24 | Thermo Scientific (from Fisher) | 66380 |
| Total Price | $78.80 |
| Total Price of E. coli crude extract, 3x Prep (Ingredients + Disposable Materials) | $141.10 |
| Price per uL of reaction | $0.00448 |
| Price per 12 uL reaction | $0.05376 |
