Biomolecular Breadboards:pBEST OR2 OR1 Pr UTR-deGFP-T500.gb: Difference between revisions
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* Control plasmid file: [[Media:pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb|pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb]] (GenBank format) | * Control plasmid file: [[Media:pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb|pBEST_OR2_OR1_Pr_UTR-deGFP-T500.gb]] (GenBank format) | ||
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'''Figure 1. eGFP expression as a function of plasmid DNA template.''' Plasmid DNA pBEST-OR2-OR1-Pr-UTR1-deGFP-T500 is varied by concentration. | '''Figure 1. eGFP expression as a function of plasmid DNA template.''' Plasmid DNA pBEST-OR2-OR1-Pr-UTR1-deGFP-T500 is varied by concentration. | ||
Revision as of 20:35, 16 July 2012
This page contains a description of the deGFP control plasmid for the cell-free expression breadboard.
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Plasmid description
Preliminary data
Using pBEST-OR2-OR1-Pr-UTR1-deGFP-T500, a plasmid enhanced for GFP expression, the biomolecular breadboard is able to express mass at equal concentrations to comparable bacteriophage in-vitro systems (J. Shin and V. Noireaux, 2010).
Expression of plasmids can be optimized by concentration.
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Figure 1. eGFP expression as a function of plasmid DNA template. Plasmid DNA pBEST-OR2-OR1-Pr-UTR1-deGFP-T500 is varied by concentration.
