Bioreactor Parameters

Biological (cells)

Medium parameters

• Media formula
• pH requirements
• Medium change

Cell parameters

• Generation time
• Cell size
• Cell density
• Swimming
• Senescence

Environmental conditions

• Temperature
• Light levels
• Gas exchange (mech. also)
• Shear stress (mech. also)

Kinetics

• hydrogenases
• nitrogenases

Mechanical (bioreactor)

Reactor design

• Vessel type
• Valving, fittings, and tubing
• Sealing/gaskets

Circulation during phases

• Pumps
• Gas diffuser
• Gas exchange (bio. also)
• Shear stress (bio. also)
• Intermittent circulation

Transition from aerobic to anaerobic/sulfur stress conditions

• Strainer, plunger

Hydrogen harvest

• Temporary gas storage/bypass
• Gas delivery to fuel cell (electrical also)

Electrical (fuel cell)

• Power consumption
• Theoretical Power generation
• Fuel cell type
• Membrane type
• Real time measurement of output/power
• Gas delivery to fuel cell (mech also)
• Gas purification
• Power from fuel cell to pump
• Charge battery

Mini-Experiments

• Strain cells
• Settling time
• Gas chromatograph of headspace