Bitan:ELISA: Difference between revisions
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0.293 g NaHCO3 (anhydrous) | 0.293 g NaHCO3 (anhydrous) | ||
0.159 g Na2CO3 (anhydrous) | 0.159 g Na2CO3 (anhydrous) | ||
*Use carbonate/bicarbonate to adjust pH to 9.6* | |||
===Blocking Buffer=== | ===Blocking Buffer=== | ||
2% Amersham ECL Advance Blocking Reagent in PBST | 2% Amersham ECL Advance Blocking Reagent in PBST | ||
| Line 17: | Line 17: | ||
1.3 g citric acid (anhydrous) | 1.3 g citric acid (anhydrous) | ||
Dissolve in 50 ml water | Dissolve in 50 ml water | ||
*Adjust pH to 5.0* | |||
*Add 40 μl fresh 30% H2O2 to 100 ml solution before use* | |||
==Reagents== | ==Reagents== | ||
Monoclonal 6E10 | Monoclonal 6E10 | ||
Revision as of 15:03, 12 November 2009
This ELISA protocol for Aβ concentration works with soluble oligomers and fibrils of Aβ(40) and Aβ(42).
Ingredients
Buffers
Coating Buffer
Carbonate/bicarbonate (pH 9.6) 100 ml 0.293 g NaHCO3 (anhydrous) 0.159 g Na2CO3 (anhydrous)
- Use carbonate/bicarbonate to adjust pH to 9.6*
Blocking Buffer
2% Amersham ECL Advance Blocking Reagent in PBST
Phosphate/Citrate Buffer
50 mM citric acid and 100 mM sodium phosphate 0.74 g dibasic sodium phosphate (anhydrous) 1.3 g citric acid (anhydrous) Dissolve in 50 ml water *Adjust pH to 5.0* *Add 40 μl fresh 30% H2O2 to 100 ml solution before use*
Reagents
Monoclonal 6E10
