Bitan:ELISA

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Revision as of 14:57, 12 November 2009 by Andrew Fithian (talk | contribs) (New page: This ELISA protocol for Aβ concentration works with soluble oligomers and fibrils of Aβ(40) and Aβ(42). =Ingredients= ==Buffers== PBS ===PBST=== ===Coating Buffer=== ===Blocking Bu...)
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This ELISA protocol for Aβ concentration works with soluble oligomers and fibrils of Aβ(40) and Aβ(42).

Ingredients

Buffers

PBS

PBST

Coating Buffer

Blocking Buffer

Phosphate/Citrate Buffer

Reagents

Monoclonal 6E10

Biotinylated 4G8

Streptavidin-HRP

o-Phenylene Diamine (OPD)

1N Sulfuric Acid

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