Bitan:ELISA

This ELISA protocol for Aβ concentration works with soluble oligomers and fibrils of Aβ(40) and Aβ(42).

Ingredients

Buffers

PBS PBST (Tween-20)

Coating Buffer

Carbonate/bicarbonate (pH 9.6) 100 ml 0.293 g NaHCO3 (anhydrous) 0.159 g Na2CO3 (anhydrous)

• • Use carbonate/bicarbonate to adjust pH to 9.6**

Blocking Buffer

2% Amersham ECL Advance Blocking Reagent in PBST

Phosphate/Citrate Buffer

50 mM citric acid and 100 mM sodium phosphate 0.74 g dibasic sodium phosphate (anhydrous) 1.3 g citric acid (anhydrous) Dissolve in 50 ml water **Adjust pH to 5.0** **Add 40 μl fresh 30% H2O2 to 100 ml solution before use**

Reagents

Monoclonal 6E10