Bitan:qPCR: Difference between revisions
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(New page: Quantitative PCR uses fluorescent dye to quantify the relative difference in sequence copy number between two samples. The dye used is SYBR Green which binds to dsDNA but not to ssDNA, whe...) |
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Revision as of 13:18, 8 December 2009
Quantitative PCR uses fluorescent dye to quantify the relative difference in sequence copy number between two samples. The dye used is SYBR Green which binds to dsDNA but not to ssDNA, when comparing two qPCR reactions of a given sequence SYBR Green's signal is proportional to sequence copy number.
The Bitan lab does not have a qPCR machine. There is one in the de Vellis lab on the third floor that you can use if you get permission from Cristina Ghiani (cghiani at mednet stop ucla stop edu). You must also sign up to use the machine at least a day in advance using the calendar that hangs over the machine.
