Bitan:todo: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 52: | Line 52: | ||
===Other amyloidogenic proteins=== | ===Other amyloidogenic proteins=== | ||
[[Aggregation | [[Aggregation (ThT Fluorescence)]]<br> | ||
[[Toxicity | [[Toxicity|Toxicity]]<br> | ||
=other pages we need= | =other pages we need= | ||
Revision as of 16:48, 11 December 2009
these are experiments that the wiki does not yet have a protocol for
Experimental Procedures
Aβ preparations
- HFIP Film Preparation
- Soluble Oligomers
- Microwave-assisted peptide synthesis
- PICUP
- Fibrils/Other Proteins
- SATA conjugation to Aβ
Molecular Biology Methods for SELEX
- qPCR
- RNA biotinylation
- PCR Amplification of the Initial ssDNA Library for SELEX experiments
- Agarose gel electrophoresis
- Measuring DNA concentration by absorbance
- In-vitro transcription, labeling and G-50 purification of RNA
- Calculating specific activity of hot CTP after calibration date
- Scintillation counting using the Triathler bench-top counter
- TBE-urea-polyacrylamide gel electrophoresis and autoradiography
- Calculating the yield and specific activity of labeled RNA
Structural Studies
Protein Analysis
- ALISA
- Western Blot
- ImageJ densitometry
- dot blot
- ELISA/ELONA/α-syn-ELISA-Total/α-syn-ELISA-oligo
- Biotinylation of antibodies
Cell Biology
Other amyloidogenic proteins
Aggregation (ThT Fluorescence)
Toxicity
other pages we need
Guidelines
Lab Members
Bitan:Research
Publications
Bitan:Talks
<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:x="urn:schemas-microsoft-com:office:excel" xmlns="http://www.w3.org/TR/REC-html40">
<head> <meta name=Title content="Molecular biology"> <meta name=Keywords content=""> <meta http-equiv=Content-Type content="text/html; charset=macintosh"> <meta name=ProgId content=Word.Document> <meta name=Generator content="Microsoft Word 11"> <meta name=Originator content="Microsoft Word 11"> <link rel=File-List href="Methods%20list_files/filelist.xml"> <title>Molecular biology</title> <!--[if gte mso 9]><xml>
<o:DocumentProperties> <o:Author>HL</o:Author> <o:Template>Normal</o:Template> <o:LastAuthor>HL</o:LastAuthor> <o:Revision>2</o:Revision> <o:Created>2009-11-26T09:20:00Z</o:Created> <o:LastSaved>2009-11-26T09:25:00Z</o:LastSaved> <o:Pages>1</o:Pages> <o:Words>259</o:Words> <o:Characters>1478</o:Characters> <o:Lines>12</o:Lines> <o:Paragraphs>2</o:Paragraphs> <o:CharactersWithSpaces>1815</o:CharactersWithSpaces> <o:Version>11.1282</o:Version> </o:DocumentProperties> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings>
</xml><![endif]--><!--[if gte mso 9]><xml>
<w:WordDocument> <w:Zoom>125</w:Zoom> <w:DisplayHorizontalDrawingGridEvery>0</w:DisplayHorizontalDrawingGridEvery> <w:DisplayVerticalDrawingGridEvery>0</w:DisplayVerticalDrawingGridEvery> <w:UseMarginsForDrawingGridOrigin/> </w:WordDocument>
</xml><![endif]--> <style> <!--
/* Font Definitions */
@font-face {font-family:"Times New Roman"; panose-1:0 2 2 6 3 5 4 5 2 3; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:50331648 0 0 0 1 0;} @font-face {font-family:Verdana; panose-1:0 2 11 6 4 3 5 4 4 2; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:50331648 0 0 0 1 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; font-size:10.0pt; font-family:Times;} a:link, span.MsoHyperlink {color:#0000D4; text-decoration:underline; text-underline:single;} a:visited, span.MsoHyperlinkFollowed {color:#993366; text-decoration:underline; text-underline:single;} table.MsoNormalTable {mso-style-parent:""; font-size:10.0pt; font-family:"Times New Roman";} p.style0, li.style0, div.style0 {mso-style-name:style0; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; font-size:10.0pt; font-family:Times;} p.xl24, li.xl24, div.xl24 {mso-style-name:xl24; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-left-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:10.0pt; font-family:Times;} p.xl25, li.xl25, div.xl25 {mso-style-name:xl25; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:10.0pt; font-family:Times;} p.xl26, li.xl26, div.xl26 {mso-style-name:xl26; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-left-alt:solid #000090 1.5pt; mso-border-bottom-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:10.0pt; font-family:Times;} p.xl27, li.xl27, div.xl27 {mso-style-name:xl27; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-bottom-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:10.0pt; font-family:Times;} p.xl28, li.xl28, div.xl28 {mso-style-name:xl28; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-bottom-alt:solid #000090 1.5pt; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:10.0pt; font-family:Times;} p.xl29, li.xl29, div.xl29 {mso-style-name:xl29; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-top-alt:solid #000090 1.5pt; mso-border-left-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:14.0pt; font-family:Times; font-weight:bold;} p.xl30, li.xl30, div.xl30 {mso-style-name:xl30; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-top-alt:solid #000090 1.5pt; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:12.0pt; font-family:Times;} p.xl31, li.xl31, div.xl31 {mso-style-name:xl31; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:12.0pt; font-family:Times;} p.xl32, li.xl32, div.xl32 {mso-style-name:xl32; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:12.0pt; font-family:Times;} p.xl33, li.xl33, div.xl33 {mso-style-name:xl33; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-bottom-alt:solid #000090 1.5pt; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:12.0pt; font-family:Times;} p.xl34, li.xl34, div.xl34 {mso-style-name:xl34; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-bottom-alt:solid #000090 1.5pt; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:12.0pt; font-family:Times; color:black;} p.xl35, li.xl35, div.xl35 {mso-style-name:xl35; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-left-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:14.0pt; font-family:Times;} p.xl36, li.xl36, div.xl36 {mso-style-name:xl36; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-left-alt:solid #000090 1.5pt; mso-border-bottom-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:14.0pt; font-family:Times;} p.xl37, li.xl37, div.xl37 {mso-style-name:xl37; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; font-size:14.0pt; font-family:Times; font-weight:bold;} p.xl38, li.xl38, div.xl38 {mso-style-name:xl38; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-top-alt:solid #000090 1.5pt; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:12.0pt; font-family:Times;} p.xl39, li.xl39, div.xl39 {mso-style-name:xl39; margin-right:0in; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; border:none; mso-border-bottom-alt:solid #000090 1.5pt; mso-border-right-alt:solid #000090 1.5pt; padding:0in; mso-padding-alt:0in 0in 0in 0in; font-size:12.0pt; font-family:Times;} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.25in 1.0in 1.25in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style> <!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1027"/>
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit"> <o:idmap v:ext="edit" data="1"/> </o:shapelayout></xml><![endif]-->
</head>
<body bgcolor=white lang=EN-US link="#0000d4" vlink="#993366" style='tab-interval: .5in'>
<div class=Section1>
<table border=0 cellspacing=0 cellpadding=0 width=721 bgcolor="#ccffff"
style='width:720.6pt;margin-left:3.0pt;background:#CCFFFF;border-collapse: collapse;mso-padding-alt:0in 0in 0in 0in'> <tr style='height:19.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Molecular biology<o:p></o:p></b></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Agarose gel electrophoresis<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Biology<o:p></o:p></b></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Barnes maze<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>TBE-urea gel electrophoresis<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Y-maze<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Autoradiography of hot blots<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Morris water maze<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Autoradiography of hot gels<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Novel object recognition<o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Calculation of RNA and DNA concentration by absorbance<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Preparation of tweezers for subcutaneous pumps<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Calculation of hot RNA specific activity and yield<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Oral gavage<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Desalting of PICUP LMW Aβ40<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Paraffin embedding<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Dot blotting<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Subcutaneous pump surgery<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>ELISA<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Immunohistochemistry<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>ELONA<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>In vitro transcription<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Elution of RNA from columns<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Intracerebroventricular pump surgery<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Elution of RNA from filters<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Intravenous and subcutaneous injection<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Exonuclease reactions for ssDNA for SELEX<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Brain protein extraction<o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Filter binding<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Mouse sacrifice, blood collection, and perfusion<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Hexokinase reactions<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Tissue ectioning and mounting<o:p></o:p></span></p> </td> </tr> <tr style='height:48.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Qiagen PCR product purification and measurement of DNA concentration, agarose electrophoresis<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Primary rat neurons<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>qPCR/ALISA<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Dendritic spine morphology<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Reverse transcription<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>TUNEL staining<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>RNA and DNA labeling<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>MTT<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>RNA extraction, precipitation, and purification<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>LDH<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Western blot<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Cell_Culture">Basic PC12 cell culture</a><o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Use of Denville intensifying screens during autoradiography<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Scintillation counting using Triathler<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>PCR and use of specific primers for either SELEX methods, RNA or DNA SELEX<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:19.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>SATA conjugation to Aβ<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:19.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Biophysics<o:p></o:p></b></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Electron_microscopy">Electron Microscopy</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Peptide Synthesis<o:p></o:p></b></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Microwave-assistant_peptide_synthesis">Microwave-assisted peptide synthesis</a><o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:ThioflavinT_fluorescence">ThT fluorescence</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>CD<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Dynamic_Light_Scattering">Dynamic light scattering</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>PICUP<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Image analysis (image J)<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:HFIP_Film_Preparation">HFIP treatment</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:33.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana;color:black'>Estimation of methionine sulfoxide reductase (Msr) activity by HPLC<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr>
</table>
<p class=MsoNormal><![if !supportEmptyParas]> <![endif]><o:p></o:p></p>
</div>
</body>
</html>
