Bitan:todo: Difference between revisions
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*[[Bitan:Calculating the yield and specific activity of labeled RNA|Calculating the yield and specific activity of labeled RNA]]<br> | *[[Bitan:Calculating the yield and specific activity of labeled RNA|Calculating the yield and specific activity of labeled RNA]]<br> | ||
*[[Bitan:Filter binding for SELEX|Filter binding for SELEX]]<br> | *[[Bitan:Filter binding for SELEX|Filter binding for SELEX]]<br> | ||
*[[Bitan:Extraction of RNA from filters|Extraction of RNA from filters]]<br> | |||
*[[Bitan:Autoradiography of hot blots|Autoradiography of hot blots]]<br> | *[[Bitan:Autoradiography of hot blots|Autoradiography of hot blots]]<br> | ||
*[[Bitan:Autoradiography of hot gels|Autoradiography of hot gels]]<br> | *[[Bitan:Autoradiography of hot gels|Autoradiography of hot gels]]<br> | ||
Revision as of 16:31, 18 December 2009
these are experiments that the wiki does not yet have a protocol for
Experimental Procedures
Aβ preparations
- HFIP Film Preparation
- Soluble Oligomers
- Microwave-assisted peptide synthesis
- PICUP
- SATA conjugation to Aβ
Molecular Biology Methods for SELEX
- qPCR
- RNA biotinylation
- PCR Amplification of the Initial ssDNA Library for SELEX experiments
- Agarose gel electrophoresis
- Measuring DNA concentration by absorbence
- In-vitro transcription, labeling and G-50 purification of RNA
- Calculating specific activity of hot CTP after calibration date
- Scintillation counting using the Triathler bench-top counter
- TBE-urea-polyacrylamide gel electrophoresis and autoradiography
- Calculating the yield and specific activity of labeled RNA
- Filter binding for SELEX
- Extraction of RNA from filters
- Autoradiography of hot blots
- Autoradiography of hot gels
Structural Studies
Protein Analysis
- ALISA
- Western Blot
- ImageJ densitometry
- dot blot
- ELISA/ELONA/α-syn-ELISA-Total/α-syn-ELISA-oligo
- Biotinylation of mAb211
Cell Biology
Other amyloidogenic proteins
Aggregation (ThT Fluorescence & EM)
Some Useful Software Usage Tips
other pages we need
Guidelines
Lab Members
Bitan:Research
Publications
Bitan:Talks
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<table border=0 cellspacing=0 cellpadding=0 width=721 bgcolor="#ccffff"
style='width:720.6pt;margin-left:3.0pt;background:#CCFFFF;border-collapse: collapse;mso-padding-alt:0in 0in 0in 0in'> <tr style='height:19.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Molecular biology<o:p></o:p></b></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Agarose gel electrophoresis<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Biology<o:p></o:p></b></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Barnes maze<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>TBE-urea gel electrophoresis<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Y-maze<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Autoradiography of hot blots<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Morris water maze<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Autoradiography of hot gels<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Novel object recognition<o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Calculation of RNA and DNA concentration by absorbance<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Preparation of tweezers for subcutaneous pumps<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Calculation of hot RNA specific activity and yield<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Oral gavage<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Desalting of PICUP LMW Aβ40<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Paraffin embedding<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Dot blotting<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Subcutaneous pump surgery<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>ELISA<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Immunohistochemistry<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>ELONA<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>In vitro transcription<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Elution of RNA from columns<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Intracerebroventricular pump surgery<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Elution of RNA from filters<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Intravenous and subcutaneous injection<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Exonuclease reactions for ssDNA for SELEX<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Brain protein extraction<o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Filter binding<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Mouse sacrifice, blood collection, and perfusion<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Hexokinase reactions<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Tissue ectioning and mounting<o:p></o:p></span></p> </td> </tr> <tr style='height:48.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Qiagen PCR product purification and measurement of DNA concentration, agarose electrophoresis<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:48.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Primary rat neurons<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>qPCR/ALISA<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Dendritic spine morphology<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Reverse transcription<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>TUNEL staining<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>RNA and DNA labeling<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>MTT<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>RNA extraction, precipitation, and purification<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>LDH<o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Western blot<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Cell_Culture">Basic PC12 cell culture</a><o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Use of Denville intensifying screens during autoradiography<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:.25in'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Scintillation counting using Triathler<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:.25in'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:32.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>PCR and use of specific primers for either SELEX methods, RNA or DNA SELEX<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:32.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:19.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>SATA conjugation to Aβ<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:19.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Biophysics<o:p></o:p></b></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Electron_microscopy">Electron Microscopy</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'><b>Peptide Synthesis<o:p></o:p></b></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:19.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Microwave-assistant_peptide_synthesis">Microwave-assisted peptide synthesis</a><o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:ThioflavinT_fluorescence">ThT fluorescence</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>CD<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:Dynamic_Light_Scattering">Dynamic light scattering</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>PICUP<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'>Image analysis (image J)<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:16.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'><a href="http://openwetware.org/wiki/Bitan:HFIP_Film_Preparation">HFIP treatment</a><o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><![if !supportEmptyParas]> <![endif]><span style='font-size:12.0pt;font-family:Verdana'><o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:16.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr> <tr style='height:33.0pt'> <td width=145 nowrap valign=bottom style='width:144.6pt;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana;color:black'>Estimation of methionine sulfoxide reductase (Msr) activity by HPLC<o:p></o:p></span></p> </td> <td width=144 nowrap valign=bottom style='width:2.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-size:12.0pt;font-family:Verdana'> <o:p></o:p></span></p> </td> <td width=216 nowrap valign=bottom style='width:3.0in;padding:1.0pt 1.0pt 0in 1.0pt; height:33.0pt'> <p class=MsoNormal style='margin:0in;margin-bottom:.0001pt'><span style='font-family:Verdana'> <o:p></o:p></span></p> </td> </tr>
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