Bitan:todo

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Revision as of 19:31, 18 December 2009 by Farid Rahimi (Talk | contribs)
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these are experiments that the wiki does not yet have a protocol for

Contents

Experimental Procedures

Aβ preparations

Molecular Biology Methods for SELEX

Structural Studies

Protein Analysis

Cell Biology

Other amyloidogenic proteins

Aggregation (ThT Fluorescence & EM)

Fibrils


Toxicity

Some Useful Software Usage Tips

other pages we need

Guidelines
Lab Members
Bitan:Research
Publications
Bitan:Talks

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Molecular biology

Molecular biology

Agarose gel electrophoresis

Biology

Barnes maze

 

TBE-urea gel electrophoresis

 

Y-maze

 

Autoradiography of hot blots

 

Morris water maze

 

Autoradiography of hot gels

 

Novel object recognition

 

Calculation of RNA and DNA concentration by absorbance

 

Preparation of tweezers for subcutaneous pumps

 

Calculation of hot RNA specific activity and yield

 

Oral gavage

 

Desalting of PICUP LMW Aβ40

 

Paraffin embedding

 

Dot blotting

 

Subcutaneous pump surgery

 

ELISA

 

Immunohistochemistry

 

ELONA

 

In vitro transcription

 

Elution of RNA from columns

 

Intracerebroventricular pump surgery

 

Elution of RNA from filters

 

Intravenous and subcutaneous injection

 

Exonuclease reactions for ssDNA for SELEX

 

Brain protein extraction

 

Filter binding

 

Mouse sacrifice, blood collection, and perfusion

 

Hexokinase reactions

 

Tissue ectioning and mounting

 

Qiagen PCR product purification and measurement of DNA concentration, agarose electrophoresis

 

Primary rat neurons

 

qPCR/ALISA

 

Dendritic spine morphology

 

Reverse transcription

 

TUNEL staining

 

RNA and DNA labeling

 

MTT

 

RNA extraction, precipitation, and purification

 

LDH

 

Western blot

 

Basic PC12 cell culture

 

Use of Denville intensifying screens during autoradiography

 

 

 

Scintillation counting using Triathler

 

 

 

PCR and use of specific primers for either SELEX methods, RNA or DNA SELEX

 

 

 

SATA conjugation to Aβ

 

 

Biophysics

Electron Microscopy

Peptide Synthesis

Microwave-assisted peptide synthesis

 

ThT fluorescence

 

 

 

CD

 

 

 

Dynamic light scattering

 

 

 

PICUP

 

 

 

Image analysis (image J)

 

 

 

HFIP treatment

 

 

 

Estimation of methionine sulfoxide reductase (Msr) activity by HPLC

 

 

 

Personal tools