Blackburn:Yeast Colony PCR: Difference between revisions
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==Materials== | ==Materials== | ||
*Standard PCR machine, tubes | *Standard PCR machine, tubes*Qiagen Taq with Q-solution | ||
*Qiagen Taq with Q-solution | *[[http://www1.qiagen.com/Products/Pcr/TaqSystem/TaqDnaPolymerase.aspx|Qiagen Taq Polymerase Kit with Q-solution]] | ||
*A small yeast colony | *A small yeast colony | ||
*0.02M NaOH | *0.02M NaOH (3uL per reaction) | ||
==Procedure== | ==Procedure== |
Revision as of 21:00, 11 June 2006
Overview
This is a quick and easy yeast colony PCR protocol that does not require zymolyase step.
Materials
- Standard PCR machine, tubes*Qiagen Taq with Q-solution
- [Taq Polymerase Kit with Q-solution]
- A small yeast colony
- 0.02M NaOH (3uL per reaction)
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding ('''~~~~''') to the end of your tip.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?