Blackburn:Yeast Colony PCR
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*Standard PCR machine, tubes*Qiagen Taq with Q-solution | *Standard PCR machine, tubes*Qiagen Taq with Q-solution | ||
| - | *[http://www1.qiagen.com/Products/Pcr/TaqSystem/TaqDnaPolymerase.aspx | + | *[http://www1.qiagen.com/Products/Pcr/TaqSystem/TaqDnaPolymerase.aspx Qiagen Taq Polymerase Kit with Q-solution] |
*A small yeast colony | *A small yeast colony | ||
*0.02M NaOH (3uL per reaction) | *0.02M NaOH (3uL per reaction) | ||
Revision as of 00:00, 12 June 2006
Contents |
Overview
This is a quick and easy yeast colony PCR protocol that does not require zymolyase step.
Materials
- Standard PCR machine, tubes*Qiagen Taq with Q-solution
- Qiagen Taq Polymerase Kit with Q-solution
- A small yeast colony
- 0.02M NaOH (3uL per reaction)
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding ('''~~~~''') to the end of your tip.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. . pmid:6947258.
- JACOB F and MONOD J. . pmid:13718526.
- Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164.
Contact
- Who has experience with this protocol?
