Blackburn:Yeast Colony PCR
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List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate. | List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate. | ||
| - | * | + | *Standard PCR machine, tubes |
| - | * | + | *Qiagen Taq with Q-solution |
| - | * | + | *A small yeast colony |
| - | + | *0.02M NaOH | |
| - | * | + | |
| - | + | ||
| - | + | ||
==Procedure== | ==Procedure== | ||
Revision as of 23:54, 11 June 2006
Contents |
Overview
This is a quick and easy yeast colony PCR protocol that does not require zymolyase step.
Materials
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.
- Standard PCR machine, tubes
- Qiagen Taq with Q-solution
- A small yeast colony
- 0.02M NaOH
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding ('''~~~~''') to the end of your tip.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. . pmid:6947258.
- JACOB F and MONOD J. . pmid:13718526.
- Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164.
Contact
- Who has experience with this protocol?
