Brett Boghigian: Difference between revisions
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== Lab Work == | == Lab Work == | ||
=== General Information === | |||
I am currently working on enhancing and optimizing the biosynthesis of 6-deoxyerythronolide B (6-dEB), precursor to the potent antibiotic [http://en.wikipedia.org/wiki/Erythromycin erythromycin], through ''Escherichia coli''. This work combines principles of molecular biology and biochemical process engineering. I am working with a new [http://www.nbsc.com/ferm_eq/bf110.asp New Brunswick BioFlo 110 Bioreactor] for large-scale production of 6-dEB and a new [http://www.chem.agilent.com/Scripts/PCol.asp?lPage=181 Agilent 1100 Series HPLC] (high performance liquid chromatograph) equipped with an Alltech 800 Series ELSD (evaporative light scattering detector) for analysis. I am also developing a new high-throughput method for analyzing our bacterial system using my previous studies in the field of proteomics (global protein production within a given cell). In collaboration with [http://las.perkinelmer.com/ PerkinElmer Life & Analytical Sciences], we will use various techniques and instruments (2D-PAGE, fluorescent protein stains, MALDI-OTOF mass spectrometry, and others) to examine the proteome of engineered versus native ''E. coli''. From quantitative proteomic data, we can reveal which native proteins play a key role during natural product biosynthesis and will give insight into the molecular and cellular aspects to be engineered for further enhanced biosynthesis. | I am currently working on enhancing and optimizing the biosynthesis of 6-deoxyerythronolide B (6-dEB), precursor to the potent antibiotic [http://en.wikipedia.org/wiki/Erythromycin erythromycin], through ''Escherichia coli''. This work combines principles of molecular biology and biochemical process engineering. I am working with a new [http://www.nbsc.com/ferm_eq/bf110.asp New Brunswick BioFlo 110 Bioreactor] for large-scale production of 6-dEB and a new [http://www.chem.agilent.com/Scripts/PCol.asp?lPage=181 Agilent 1100 Series HPLC] (high performance liquid chromatograph) equipped with an Alltech 800 Series ELSD (evaporative light scattering detector) for analysis. I am also developing a new high-throughput method for analyzing our bacterial system using my previous studies in the field of proteomics (global protein production within a given cell). In collaboration with [http://las.perkinelmer.com/ PerkinElmer Life & Analytical Sciences], we will use various techniques and instruments (2D-PAGE, fluorescent protein stains, MALDI-OTOF mass spectrometry, and others) to examine the proteome of engineered versus native ''E. coli''. From quantitative proteomic data, we can reveal which native proteins play a key role during natural product biosynthesis and will give insight into the molecular and cellular aspects to be engineered for further enhanced biosynthesis. | ||
=== Current Projects === | |||
* Examining the effect of the SAM gene on 6-deoxyerythronolide B biosynthesis in engineered ''Escherichia coli''. | |||
* Proteomics in natural products research: quantitative, high-throughput, comparative proteomics of engineered ''Escherichia coli'' for biosynthesis of 6-deoxyerythronolide B. | |||
== Academic & Research Interests == | == Academic & Research Interests == | ||
Revision as of 17:05, 15 January 2006
Lab Work
General Information
I am currently working on enhancing and optimizing the biosynthesis of 6-deoxyerythronolide B (6-dEB), precursor to the potent antibiotic erythromycin, through Escherichia coli. This work combines principles of molecular biology and biochemical process engineering. I am working with a new New Brunswick BioFlo 110 Bioreactor for large-scale production of 6-dEB and a new Agilent 1100 Series HPLC (high performance liquid chromatograph) equipped with an Alltech 800 Series ELSD (evaporative light scattering detector) for analysis. I am also developing a new high-throughput method for analyzing our bacterial system using my previous studies in the field of proteomics (global protein production within a given cell). In collaboration with PerkinElmer Life & Analytical Sciences, we will use various techniques and instruments (2D-PAGE, fluorescent protein stains, MALDI-OTOF mass spectrometry, and others) to examine the proteome of engineered versus native E. coli. From quantitative proteomic data, we can reveal which native proteins play a key role during natural product biosynthesis and will give insight into the molecular and cellular aspects to be engineered for further enhanced biosynthesis.
Current Projects
- Examining the effect of the SAM gene on 6-deoxyerythronolide B biosynthesis in engineered Escherichia coli.
- Proteomics in natural products research: quantitative, high-throughput, comparative proteomics of engineered Escherichia coli for biosynthesis of 6-deoxyerythronolide B.
Academic & Research Interests
My academic and research interests lie in integrating molecular biology and biochemistry with engineering techniques for a broad-range of clinical applications. Specific interests and current projects:
- Natural product biosynthesis: Cellular and process engineering of Escherichia coli for biosynthesis of complex medicinal compounds
- Metabolic engineering: High-throughput proteomics and "omics" technologies aiding in the fields of metabolic and cellular engineering
- Systems biology: The development of high-throughput tools and methodologies for examining systems from the systems to the cellular to the molecular level
- Tissue engineering: Drug delivery systems to promote angiogenesis in engineered tissues and cardiac tissue engineering
- Philosophy: Biological macromolecular evolution and philosophy of molecular biology
Personal
I am an undergraduate pursuing a bachelor's of science in chemical engineering with a second major of biomedical engineering. I am also minoring in philosophy. If not evident from my native accent, I grew up in a suburb of Boston: Bedford, MA and attended Bedford High School. I enjoy hanging out with my friends, watching movies (The Godfather and Scarface, in particular), watching the Patriots and Red Sox, playing sports (tennis, golf, ping-pong, running, baseball, and basketball), and listening to music.
