Brett Boghigian: Difference between revisions

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[[Pfeifer Lab|Pfeifer Lab OWW Homepage]]
[[Image:2009Sumposium2.jpg|thumb|500px|right|Giving a talk at Tufts in the spring of 2009]]
[[Image:brett.jpg|thumb|300px|right|That's me.]]
== Contact Information ==
== Lab Work ==
In April 2011, Brett joined the founding team as the third scientific hire of [http://www.pronutria.com/ Pronutria] (formerly known as Essentient, Inc.).  Pronutria is a [http://www.flagshipventures.com/venturelabs Flagship VentureLabs™] company, founded to transform global food security and nutrition, fueling human potential.


=== General Information ===
== Education ==
I am currently working on enhancing and optimizing the biosynthesis of 6-deoxyerythronolide B (6-dEB), precursor to the potent antibiotic [http://en.wikipedia.org/wiki/Erythromycin erythromycin], through ''Escherichia coli'', the workhorse of the bacterial world. This work combines principles of molecular biology and biochemical process engineering. I am working with a new [http://www.nbsc.com/ferm_eq/bf110.asp New Brunswick BioFlo 110 Bioreactor] for large-scale production of 6-dEB and a new [http://www.chem.agilent.com/Scripts/PCol.asp?lPage=181 Agilent 1100 Series HPLC] (high performance liquid chromatograph) equipped with an [http://www.alltechweb.com/productinfo/technical/datasheets/M073.pdf Alltech 800 Series ELSD] (evaporative light scattering detector) for analysis. I am also developing a new high-throughput method for analyzing our bacterial system using my studies in the field of proteomics (global protein production within a given cell or tissue). In collaboration with Dr. Mary Lopez and Dr. Wayne Patton at [http://las.perkinelmer.com/ PerkinElmer Life & Analytical Sciences], we will use various proteomic techniques and instruments (2D SDS-PAGE, [http://las.perkinelmer.com/Catalog/default.htm?CategoryID=Imaging+%26+Analysis fluorescent protein staining and imaging], [http://las.perkinelmer.com/Content/RelatedMaterials/006860-prTOF2000MALDIO-TOF-SpecificationsSheet.pdf MALDI O-TOF mass spectrometry], and others) to examine the proteome various engineered strains versus our parent strain of E. coli. Using quantitative proteomic data, we can identify which proteins are down- or up-regulated during certain intracellular conditions (ie. insertion of new plasmid DNA) or extracellular conditions (ie. altered bioprocess conditions), which can be exploited for further enhanced natural product biosynthesis. I am also responsible for website maintenance and our lab’s (frequent) computer problems.
* 2010 - Ph.D. Chemical Engineering, Tufts University
**Thesis: "Multi-scale engineering and modeling of heterologous natural product biosynthesis in ''Escherichia coli''" (384pp)
**Adviser: [http://www.cbe.buffalo.edu/people/full_time/b_pfeifer.php Professor Blaine A. Pfeifer]
* 2007 - B.S. Chemical Engineering (Minor in Philosophy), Tufts University


=== Projects (Current & Pending) ===
==Scientific Expertise==
* Examining the effect of the introduction of the gene coding for S-adenosylmethionine synthetase (SAMs) in engineered ''Escherichia coli'' on 6-deoxyerythronolide B biosynthesis.
Metabolic engineering and strain development, upstream and downstream bioprocess development, photosynthetic organisms, computational systems biology, analytical chemistry, natural products, synthetic biology
* Proteomics aiding in natural product biosynthesis -- an application to ''Escherichia coli'' for enhancing 6-deoxyerythronolide B biosynthesis.
* Inverse metabolic engineering approach for enhancing biosynthesis of 6-deoxyerythronolide B in engineered ''Escherichia coli''.


===Publications===
==Peer-Reviewed Publications==
''Peer-Reviewed Publications:''<br>* Scrivener E, '''Boghigian BA''', Golenko E, Bogdanova A, Jackson P, Mikulskis A, Denoyer E, Courtney P, Lopez MF, Patton WF. Performance validation of an improved Xenon-arc lamp-based CCD camera system for multispectral imaging in proteomics. ''Proteomics.'' 2005 Nov;5(17):4354-66. ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16206330&query_hl=7&itool=pubmed_docsum PubMed]) (Times Cited: 1)
(* indicates co-first author. <sup>†</sup> indicates undergraduate author)
*'''Boghigian BA''', Armando J<sup>†</sup>, Salas DF<sup>†</sup>, Pfeifer BA. Computational identification of gene over-expression targets for metabolic engineering. ''Appl Microbiol Biotechnol''. 2012 Mar;93(5):2063-73. ([http://www.springerlink.com/content/139515226x50p4l8/ Link])
*'''Boghigian BA''', Salas DF<sup>†</sup>, Ajikumar PK, Stephanopoulos G, Pfeifer BA. Analysis of heterologous taxadiene production in K- and B-derived ''Escherichia coli''. ''Appl Microbiol Biotechnol''. 2012 Feb;93(4):1651-61. ([http://www.springerlink.com/content/et466722130hk103/ Link])
*Armando J<sup>†</sup>, '''Boghigian BA''', Pfeifer BA. LC-MS/MS quantification of short-chain acyl-CoA's in ''Escherichia coli'' demonstrates versatile propionyl-CoA synthetase substrate specificity. ''Lett Appl Microbiol''. 2012 Feb;54(2):140-8. ([http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2011.03184.x/abstract Link])
*'''Boghigian BA'''*, Myint M*<sup>†</sup>, Wu J, Pfeifer BA. Simultaneous production and partitioning of heterologous polyketide and isoprenoid natural products in an ''Escherichia coli'' two-phase bioprocess. ''J Ind Microbiol Biotechnol''. 2011 Nov;38(11):1809-20. ([http://www.springerlink.com/content/3u171954p2h43786/ Link])
*'''Boghigian BA''', Zhang H, Pfeifer BA. Multi-factorial engineering of heterologous polyketide production in ''Escherichia coli'' reveals complex pathway interactions. ''Biotechnol Bioeng''. 2011 Jun;108(6):1360-71. ([http://onlinelibrary.wiley.com/doi/10.1002/bit.23069/abstract;jsessionid=41BBB7049A007C86746D07C6D4DE59E5.d02t03 Link])
*Zhang H, '''Boghigian BA''', Armando J<sup>†</sup>, Pfeifer BA. Methods and options for the heterologous production of complex natural products. ''Nat Prod Rep''. 2011 Jan 17;28(1):125-51. ([http://pubs.rsc.org/en/Content/ArticleLanding/2011/NP/c0np00037j Link])
*Wu J, '''Boghigian BA''', Myint M<sup>†</sup>, Zhang H, Zhang S, Pfeifer BA. Construction and performance of heterologous polyketide producing K-12- and B-derived ''Escherichia coli''. ''Lett Appl Microbiol''. 2010 Aug;51(2):196-204. ([http://www.ncbi.nlm.nih.gov/pubmed/20565574 PubMed])
*'''Boghigian BA''', Shi H, Lee K, Pfeifer BA. Utilizing elementary mode analysis, pathway thermodynamics, and a genetic algorithm for metabolic flux determination and optimal metabolic network design. ''BMC Syst Biol''. 2010 Apr 23;4(1):49. ([http://www.ncbi.nlm.nih.gov/pubmed/20416071 PubMed]) '''"Highly Accessed" status'''
*'''Boghigian BA''', Seth G, Kiss R, Pfeifer BA. Metabolic flux analysis and pharmaceutical production. ''Metab Eng''. 2010 Mar;12(2):81-95. ([http://www.ncbi.nlm.nih.gov/pubmed/19861167 PubMed]) '''"Most Downloaded" status'''
*Zhang H, '''Boghigian BA''', Pfeifer BA. Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in ''Escherichia coli''. ''Biotechnol Bioeng''. 2010 Feb 15;105(3):567-73. ([http://www.ncbi.nlm.nih.gov/pubmed/19806677 PubMed])
*'''Boghigian BA''', Lee K, Pfeifer BA. Computational analysis of phenotypic space in heterologous polyketide biosynthesis – applications to ''Escherichia coli'', ''Bacillus subtilis'', and ''Saccharomyces cerevisiae''. ''J Theor Biol''. 2010 Jan 21;262(2):197-207. ([http://www.ncbi.nlm.nih.gov/pubmed/19833139 PubMed])
*Zhang H, Wang Y, '''Boghigian BA''', Pfeifer BA. Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in ''E. coli''. ''Microbial Biotechnol''. 2009 May;2(3):390-4. ([http://www3.interscience.wiley.com/journal/122267706/abstract Link])
*'''Boghigian BA''', Pfeifer BA. Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in ''Escherichia coli''. ''Biotechnol Lett''. 2008 Aug;30(8):1323-30. Review. ([http://www.ncbi.nlm.nih.gov/pubmed/18488149 PubMed])
*Wang Y*, '''Boghigian BA'''*<sup>†</sup>, Pfeifer BA. Improving heterologous polyketide production in ''Escherichia coli'' by overexpression of an ''S''-adenosylmethionine synthetase gene. ''Appl Microbiol Biotechnol''. 2007 Nov;77(2):367-73. ([http://www.ncbi.nlm.nih.gov/pubmed/18488149 PubMed])
*Scrivener E, '''Boghigian BA'''<sup>†</sup>, Golenko E, Bogdanova A, Jackson P, Mikulskis A, Denoyer E, Courtney P, Lopez MF, Patton WF. Performance validation of an improved Xenon-arc lamp-based CCD camera system for multispectral imaging in proteomics. ''Proteomics''. 2005 Nov;5(17):4354-66. ([http://www.ncbi.nlm.nih.gov/pubmed/16206330 PubMed])


''Abstracts:''<br>* Stehmann C, '''Boghigian BA''', Golenko E, Scrivener E, Patton WF, Lopez MF. A Xenon-arc lamp-based charge-coupled device (CCD) camera system for multispectral imaging in proteomics. ''Mol Cell Proteomics.'' (Abstract from HUPO (Human Proteome Organization) 3rd Annual World Congress) ([[Media:HUPOposter2004.pdf|Poster]])
==Conference Presentations==
*'''Boghigian BA''', Pfeifer BA. Multi-scale engineering of heterologous polyketide and isoprenoid natural products in ''Escherichia coli''. SIM Annual Meeting (San Francisco, CA). 2010.
*'''Boghigian BA''', Wang Y, Salas D, Stephanopoulos G, Pfeifer BA. Multi-scale engineering of a Taxol<sup>®</sup> precursor in ''Escherichia coli''. AIChE Annual Meeting (Nashville, TN). 2009.
*'''Boghigian BA''', Wang Y, Pistorino M, Pfeifer BA. Multiple approaches to improving heterologous polyketide production from ''E. coli''. Metabolic Engineering VII (Puerto Vallarta, Mexico). 2008.
*'''Boghigian BA''', Wang Y, Lee K, Pfeifer BA. Improving heterologous polyketide production in ''Escherichia coli'' through signal pathway engineering and metabolic modeling. Boston Bacteriology Meeting (Cambridge, MA). 2008.
*'''Boghigian BA'''<sup>†</sup>, Wang Y, Pfeifer BA. A closer look at ''E. coli''-derived 6-deoxyerythronolide B biosynthesis. AIChE Annual Meeting (San Francisco, CA). 2006.
*Wang Y, '''Boghigian BA'''<sup>†</sup>, Shi G, Zhang H, Lavoie JR, Pfeifer BA. Enhancing polyketide production in ''E. coli'' by the accumulating of intracellular ''S''-adenosylmethionine. Boston Bacterial Meeting (Cambridge, MA). 2006.
*Stehmann C, '''Boghigian BA'''<sup>†</sup>, Golenko E, Scrivener E, Patton WF, Lopez MF. A xenon-arc lamp-based charge-coupled device (CCD) camera system for multispectral imaging in proteomics. ''Mol Cell Proteomics''. 2004 Oct;3(10):S301. HUPO 3rd Annual World Congress (Beijing, China). 2004.


== Academic & Research Interests ==
== Work Experience ==
My academic and research interests lie in integrating molecular biology and biochemistry with engineering techniques for a broad-range of clinical applications. Specific interests and current projects:
* 2011.04-current – [http://www.pronutria.com/ Pronutria] (Cambridge, MA)
* '''Natural product biosynthesis:''' Metabolic and process engineering of ''Escherichia coli'' for biosynthesis of medicinal natural products.
* 2007.09-2011.04 – Pfeifer Lab, Chemical & Biological Engineering, Tufts University (Medford, MA)
* '''Metabolic engineering:''' High-throughput proteomics and "omics" technologies aiding in the fields of metabolic and cellular engineering.
* 2007.05-2007.09 – [http://www.wyeth.com/ Wyeth BioPharma (currently Pfizer)], Purification Process Development (Andover, MA)
* '''Systems biology:''' The development of high-throughput tools and methodologies for examining systems from the systems to the metabolic to the molecular level.
* 2005.05-2007.05 – Pfeifer Lab, Chemical & Biological Engineering, Tufts University (Medford, MA)
* '''Philosophy of science:''' Biomolecular evolution, biological reductionism, philosophy of molecular biology, bioethics
* 2004.09-2005.05 – [http://ase.tufts.edu/biomedical/faculty-staff/kaplan.asp Kaplan Lab], Biomedical Engineering, Tufts University (Medford, MA)
* 2004.04-2004.09 – [http://www.perkinelmer.com/ PerkinElmer Life & Analytical Sciences], Biochemistry (Boston, MA)


== Personal ==
== Teaching Experience ==
 
(all at Tufts University)
I am an undergraduate pursuing a bachelor's of science in chemical engineering with a second major of biomedical engineering. I am also minoring in philosophy. If not evident from my native accent, I grew up in a suburb of Boston: Bedford, MA and attended Bedford High School. I enjoy hanging out with my friends, watching movies ([http://www.imdb.com/title/tt0068646/ ''The Godfather''] and [http://www.imdb.com/title/tt0086250/ ''Scarface''], in particular), watching the [http://www.patriots.com/ Patriots] and [http://boston.redsox.mlb.com/NASApp/mlb/index.jsp?c_id=bos Red Sox], listening to music, and (given time) playing sports (tennis, golf, ping-pong, running, baseball, and basketball).
*Spring 2009 – ''CHBE24B – Senior Projects Laboratory'', Teaching Assistant for Prof. David R. Vinson
 
*Fall 2008 – ''CHBE24A – Senior Projects Laboratory'', Teaching Assistant for Prof. David R. Vinson
== Links ==
*Spring 2008 – ''CHBE168 – Biotechnology Process Projects Laboratory'', Teaching Assistant for [http://engineering.tufts.edu/chbe/people/yi/index.asp Prof. Hyunmin Yi]
===About Me===
*Spring 2008 – ''CHBE24B – Senior Projects Laboratory'', Teaching Assistant for [http://www.cbe.buffalo.edu/people/full_time/b_pfeifer.php Prof. Blaine A. Pfeifer]
* [http://www.tuftsdaily.com/media/storage/paper856/news/2006/03/13/News/Showcasing.Research.From.squishies.To.Snails-1684694.shtml?norewrite200604221924&sourcedomain=www.tuftsdaily.com Article/Picture in the Tufts Daily (3/13/06) - 2006 Undergraduate Research Symposium]
*Fall 2007 – ''CHBE10 – Thermodynamics & Process Calculations I'', Guest Lecturer for [http://www.cbe.buffalo.edu/people/full_time/b_pfeifer.php Prof. Blaine A. Pfeifer]
* [http://ase.tufts.edu/chemical/facPfeiferStuBoghigian.htm On the CHBE Department website]
*Fall 2007 – ''CHBE24A − Senior Projects Laboratory'', Teaching Assistant for [http://www.cbe.buffalo.edu/people/full_time/b_pfeifer.php Prof. Blaine A. Pfeifer]
* [http://engineering.tufts.edu/profile-students.asp On the School of Engineering Website]
*Fall 2006 – ''EN3 – Introduction to Biotechnology Engineering'', Guest Lecturer for [http://engineering.tufts.edu/chbe/people/kaplan/index.asp Prof. David L. Kaplan]
 
===Departmental Websites===
* [http://www.tufts.edu/main.php?p=flash Tufts University]
* [http://ase.tufts.edu/chemical/facPfeifer.htm Tufts University - The Pfeifer Lab]
* [http://engineering.tufts.edu/ Tufts University - School of Engineering]
* [http://ase.tufts.edu/chemical/ Tufts University - Department of Chemical & Biological Engineering]
* [http://ase.tufts.edu/biomedical/ Tufts University - Department of Biomedical Engineering]
* [http://chem.tufts.edu/ Tufts University - Department of Chemistry]
* [http://ase.tufts.edu/philosophy/ Tufts University - Department of Philosophy]
* [http://web.mit.edu/cheme/ Massachusetts Institute of Technology - Department of Chemical Engineering]
* [http://web.mit.edu/be/index.htm Massachusetts Institute of Technology - Biological Engineering Division]
* [http://web.mit.edu/biology/www/ Massachusetts Institute of Technology - Department of Biology]
* [http://www.chem.harvard.edu/ Harvard University - Department of Chemistry & Chemical Biology]
* [http://www.deas.harvard.edu/research/bioengineering.html Harvard University - Division of Engineering & Applied Sciences (Bioengineering)]
* [http://www.cheme.cornell.edu/ Cornell University - School of Chemical & Biomolecular Engineering]
* [http://chemeng.stanford.edu/ Stanford University - Department of Chemical Engineering]
* [http://cheme.berkeley.edu/ University of California at Berkeley - Department of Chemical Engineering]
* [http://www.che.caltech.edu/ California Institute of Technology - Department of Chemical Engineering]
* [http://www.wi.mit.edu/ The Whitehead Institute for Biomedical Research]
* [http://www.broad.mit.edu/ The Broad Institute]
* [http://www.hhmi.org/ The Howard Hughes Medical Institute]
 
===Labs of Interest===
* [http://web.mit.edu/bamel/index.shtml Gregory Stephanopoulos] (MIT)
* [http://web.mit.edu/kdw-lab/ K. Dane Wittrup] (MIT)
* [http://web.mit.edu/dicwanggroup/index.shtml Daniel I.C. Wang] (MIT)
* [http://ase.tufts.edu/chemical/facLee.htm Kyongum Lee] (Tufts)
* [http://web.mit.edu/cbe/dallab/doug.htm Doug Lauffenburger] (MIT)
* [http://openwetware.org/wiki/Endy_Lab Drew Endy] (MIT)
* [http://web.mit.edu/be/people/tidor.htm Bruce Tidor] (MIT)
* [http://web.mit.edu/be/people/alm.htm Eric Alm] (MIT)
* [http://www.leelab.org/ Kelvin Lee] (Cornell)
* [http://www.cchem.berkeley.edu/~jdkgrp/ Jay Kesling] (UC Berkeley)
* [http://www.stanford.edu/dept/chemistry/faculty/khosla/ Chaitan Khosla] (Stanford)
* [http://www.che.caltech.edu/groups/cds/index.htm Christina Smolke] (CalTech)
* [http://www.che.caltech.edu/faculty/arnold_f/index.html Frances Arnold] (CalTech)
* [http://www.chem.brown.edu/faculty/dec/resgrp/ David Cane] (Brown)
* [http://gmwgroup.harvard.edu/ George Whitesides] (Harvard)
* [http://www.cgr.harvard.edu/macbeath/ Gavin MacBeath] (Harvard)
* [http://people.deas.harvard.edu/~dedwards/ David Edwards] (Harvard)
* [http://arep.med.harvard.edu/ George Church] (Harvard)
* [http://walsh.med.harvard.edu/ Christopher Walsh] (Harvard)
 
[[Pfeifer Lab|Pfeifer Lab OWW Homepage]]

Latest revision as of 18:40, 2 January 2013

Giving a talk at Tufts in the spring of 2009

Contact Information

In April 2011, Brett joined the founding team as the third scientific hire of Pronutria (formerly known as Essentient, Inc.). Pronutria is a Flagship VentureLabs™ company, founded to transform global food security and nutrition, fueling human potential.

Education

  • 2010 - Ph.D. Chemical Engineering, Tufts University
    • Thesis: "Multi-scale engineering and modeling of heterologous natural product biosynthesis in Escherichia coli" (384pp)
    • Adviser: Professor Blaine A. Pfeifer
  • 2007 - B.S. Chemical Engineering (Minor in Philosophy), Tufts University

Scientific Expertise

Metabolic engineering and strain development, upstream and downstream bioprocess development, photosynthetic organisms, computational systems biology, analytical chemistry, natural products, synthetic biology

Peer-Reviewed Publications

(* indicates co-first author. † indicates undergraduate author)

  • Boghigian BA, Armando J†, Salas DF†, Pfeifer BA. Computational identification of gene over-expression targets for metabolic engineering. Appl Microbiol Biotechnol. 2012 Mar;93(5):2063-73. (Link)
  • Boghigian BA, Salas DF†, Ajikumar PK, Stephanopoulos G, Pfeifer BA. Analysis of heterologous taxadiene production in K- and B-derived Escherichia coli. Appl Microbiol Biotechnol. 2012 Feb;93(4):1651-61. (Link)
  • Armando J†, Boghigian BA, Pfeifer BA. LC-MS/MS quantification of short-chain acyl-CoA's in Escherichia coli demonstrates versatile propionyl-CoA synthetase substrate specificity. Lett Appl Microbiol. 2012 Feb;54(2):140-8. (Link)
  • Boghigian BA*, Myint M*†, Wu J, Pfeifer BA. Simultaneous production and partitioning of heterologous polyketide and isoprenoid natural products in an Escherichia coli two-phase bioprocess. J Ind Microbiol Biotechnol. 2011 Nov;38(11):1809-20. (Link)
  • Boghigian BA, Zhang H, Pfeifer BA. Multi-factorial engineering of heterologous polyketide production in Escherichia coli reveals complex pathway interactions. Biotechnol Bioeng. 2011 Jun;108(6):1360-71. (Link)
  • Zhang H, Boghigian BA, Armando J†, Pfeifer BA. Methods and options for the heterologous production of complex natural products. Nat Prod Rep. 2011 Jan 17;28(1):125-51. (Link)
  • Wu J, Boghigian BA, Myint M†, Zhang H, Zhang S, Pfeifer BA. Construction and performance of heterologous polyketide producing K-12- and B-derived Escherichia coli. Lett Appl Microbiol. 2010 Aug;51(2):196-204. (PubMed)
  • Boghigian BA, Shi H, Lee K, Pfeifer BA. Utilizing elementary mode analysis, pathway thermodynamics, and a genetic algorithm for metabolic flux determination and optimal metabolic network design. BMC Syst Biol. 2010 Apr 23;4(1):49. (PubMed) "Highly Accessed" status
  • Boghigian BA, Seth G, Kiss R, Pfeifer BA. Metabolic flux analysis and pharmaceutical production. Metab Eng. 2010 Mar;12(2):81-95. (PubMed) "Most Downloaded" status
  • Zhang H, Boghigian BA, Pfeifer BA. Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in Escherichia coli. Biotechnol Bioeng. 2010 Feb 15;105(3):567-73. (PubMed)
  • Boghigian BA, Lee K, Pfeifer BA. Computational analysis of phenotypic space in heterologous polyketide biosynthesis – applications to Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae. J Theor Biol. 2010 Jan 21;262(2):197-207. (PubMed)
  • Zhang H, Wang Y, Boghigian BA, Pfeifer BA. Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in E. coli. Microbial Biotechnol. 2009 May;2(3):390-4. (Link)
  • Boghigian BA, Pfeifer BA. Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in Escherichia coli. Biotechnol Lett. 2008 Aug;30(8):1323-30. Review. (PubMed)
  • Wang Y*, Boghigian BA*†, Pfeifer BA. Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene. Appl Microbiol Biotechnol. 2007 Nov;77(2):367-73. (PubMed)
  • Scrivener E, Boghigian BA†, Golenko E, Bogdanova A, Jackson P, Mikulskis A, Denoyer E, Courtney P, Lopez MF, Patton WF. Performance validation of an improved Xenon-arc lamp-based CCD camera system for multispectral imaging in proteomics. Proteomics. 2005 Nov;5(17):4354-66. (PubMed)

Conference Presentations

  • Boghigian BA, Pfeifer BA. Multi-scale engineering of heterologous polyketide and isoprenoid natural products in Escherichia coli. SIM Annual Meeting (San Francisco, CA). 2010.
  • Boghigian BA, Wang Y, Salas D, Stephanopoulos G, Pfeifer BA. Multi-scale engineering of a Taxol® precursor in Escherichia coli. AIChE Annual Meeting (Nashville, TN). 2009.
  • Boghigian BA, Wang Y, Pistorino M, Pfeifer BA. Multiple approaches to improving heterologous polyketide production from E. coli. Metabolic Engineering VII (Puerto Vallarta, Mexico). 2008.
  • Boghigian BA, Wang Y, Lee K, Pfeifer BA. Improving heterologous polyketide production in Escherichia coli through signal pathway engineering and metabolic modeling. Boston Bacteriology Meeting (Cambridge, MA). 2008.
  • Boghigian BA†, Wang Y, Pfeifer BA. A closer look at E. coli-derived 6-deoxyerythronolide B biosynthesis. AIChE Annual Meeting (San Francisco, CA). 2006.
  • Wang Y, Boghigian BA†, Shi G, Zhang H, Lavoie JR, Pfeifer BA. Enhancing polyketide production in E. coli by the accumulating of intracellular S-adenosylmethionine. Boston Bacterial Meeting (Cambridge, MA). 2006.
  • Stehmann C, Boghigian BA†, Golenko E, Scrivener E, Patton WF, Lopez MF. A xenon-arc lamp-based charge-coupled device (CCD) camera system for multispectral imaging in proteomics. Mol Cell Proteomics. 2004 Oct;3(10):S301. HUPO 3rd Annual World Congress (Beijing, China). 2004.

Work Experience

  • 2011.04-current – Pronutria (Cambridge, MA)
  • 2007.09-2011.04 – Pfeifer Lab, Chemical & Biological Engineering, Tufts University (Medford, MA)
  • 2007.05-2007.09 – Wyeth BioPharma (currently Pfizer), Purification Process Development (Andover, MA)
  • 2005.05-2007.05 – Pfeifer Lab, Chemical & Biological Engineering, Tufts University (Medford, MA)
  • 2004.09-2005.05 – Kaplan Lab, Biomedical Engineering, Tufts University (Medford, MA)
  • 2004.04-2004.09 – PerkinElmer Life & Analytical Sciences, Biochemistry (Boston, MA)

Teaching Experience

(all at Tufts University)

  • Spring 2009 – CHBE24B – Senior Projects Laboratory, Teaching Assistant for Prof. David R. Vinson
  • Fall 2008 – CHBE24A – Senior Projects Laboratory, Teaching Assistant for Prof. David R. Vinson
  • Spring 2008 – CHBE168 – Biotechnology Process Projects Laboratory, Teaching Assistant for Prof. Hyunmin Yi
  • Spring 2008 – CHBE24B – Senior Projects Laboratory, Teaching Assistant for Prof. Blaine A. Pfeifer
  • Fall 2007 – CHBE10 – Thermodynamics & Process Calculations I, Guest Lecturer for Prof. Blaine A. Pfeifer
  • Fall 2007 – CHBE24A − Senior Projects Laboratory, Teaching Assistant for Prof. Blaine A. Pfeifer
  • Fall 2006 – EN3 – Introduction to Biotechnology Engineering, Guest Lecturer for Prof. David L. Kaplan
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