Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydyroxyl groups).
- agarose gel loading buffer
- agarose gel electrophoresis
- Xylene cyanol - slower than BPB
- Cresol Red - slower than BPB