Bryan Hernandez: Difference between revisions

Revision as of 15:35, 24 January 2006

Sortostat
- We are currently using strain MC4100 E.Coli expressing CFP (Cyan Fluorescent Protein, contained on plasmid pSB1A2 ) and YFP (Yellow Fluorescent Protein) as these are easily distinguished between visually thereby aiding in our descriminitive assortment without relying on chemical selection. In this way, it is less likely for cells to avoid being sorted by a genetic mutation as one might expect using an antibiotic.
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Project Goals

Sortostat

Debug a microfluidic chemostat (Sortostat) to improve the time-varying specific selection of cell populations.
- Currently troubleshooting problems: i) Cell Death after 3-4 days (presumably due to oxygen depletion,) and ii) Inaccurate cell counts due to poor image processing.
Evaluate the response of populations of E.Coli cells containing engineered genetic circuits (http://parts.mit.edu) to particular selective pressures using the Sortostat.

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 == Projects ==
 #[[Sortostat]]
-#*We are currently using strains of E.Coli expressing CFP (Cyan Fluorescent Protein) and YFP (Yellow Fluorescent Protein) as these are easily distinguished between visually thereby aiding in our descriminitive assortment without relying on chemical selection.  In this way, it is less likely for cells to avoid being sorted by a genetic mutation as one might express using an antibiotic.
+#*We are currently using strain [[MC4100]] E.Coli expressing CFP (Cyan Fluorescent Protein, contained on plasmid [http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=4659 pSB1A2] ) and YFP (Yellow Fluorescent Protein) as these are easily distinguished between visually thereby aiding in our descriminitive assortment without relying on chemical selection.  In this way, it is less likely for cells to avoid being sorted by a genetic mutation as one might expect using an antibiotic.
 #...