Bryan Hernandez: Difference between revisions
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#*Currently troubleshooting problems: i) Cell Death after 3-4 days (presumably due to oxygen depletion,) and ii) Inaccurate cell counts due to poor image processing. | #*Currently troubleshooting problems: i) Cell Death after 3-4 days (presumably due to oxygen depletion,) and ii) Inaccurate cell counts due to poor image processing. | ||
#Evaluate the response of populations of E.Coli cells containing engineered genetic circuits (http://parts.mit.edu) to particular selective pressures using the Sortostat. | #Evaluate the response of populations of E.Coli cells containing engineered genetic circuits (http://parts.mit.edu) to particular selective pressures using the Sortostat. | ||
[[UROP Proposal]] | |||
Revision as of 00:01, 14 February 2006
Bio
- I am currently a UROP in the Endy Lab working with Jason Kelly.
- Class of 2009; Majoring in Mathematics and Biological Engineering.
- NorCal=home
Projects
Sortostat
- We are currently using strain MC4100 E.Coli capable of expressing CFP and YFP (Cyan Fluorescent Protein and Yellow Fluorescent Protein, contained on plasmid pSB1A2 ) as these are easily distinguished between visually thereby aiding in our descriminitive assortment without relying on chemical selection. In this way, it is less likely for cells to avoid being sorted by a genetic mutation as one might expect using an antibiotic.
- Chemostat Theory
- Sortostat/Experiments
- Sortostat/Growth Tests
...
Project Goals
- Debug a microfluidic chemostat (Sortostat) to improve the time-varying specific selection of cell populations.
- Currently troubleshooting problems: i) Cell Death after 3-4 days (presumably due to oxygen depletion,) and ii) Inaccurate cell counts due to poor image processing.
- Evaluate the response of populations of E.Coli cells containing engineered genetic circuits (http://parts.mit.edu) to particular selective pressures using the Sortostat.
