Bryan Hernandez: Difference between revisions

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== '''Bio''' ==
For information on me, see [http://thebryanhernandezgame.wordpress.com/ The Bryan Hernandez Game].
*I am currently a UROP in the Endy Lab working with [[Jason Kelly]].
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*Class of 2009; Course 18 and BE.
== Bio ==
*NorCal=home


*I am currently an undergraduate researcher in the [[Endy Lab]] at MIT.  I am currently building and characterizing orthogonal riboregulators.  Learn more about riboregulators and the work I have done [http://parts2.mit.edu/wiki/index.php/Berkeley2006-RiboregulatorsMain here]
*MIT Class of 2009; Majoring in [http://math.mit.edu/| Mathematics] and [http://web.mit.edu/be/index.htm| Biological Engineering].


== '''Project Goals''' ==
== Projects ==
#Debug a microfluidic chemostat ([[Sortostat]]) to improve the time-varying specific selection of cell populations.  
 
#*Current problems include:
===Construction and Characterization of Riboregulators===
    ''i)''Cell Death after 3-4 days (presumably due to oxygen depletion.)
A Riboregulator is a post-transcriptional regulator used in bacteria.  Its mechanism for regulation involves the occlusion of the Ribosome via a hairpin on the mRNA transcript in the 'off' state thereby preventing (or 'locking') translation of the ORF.  The 'on' state is recovered by introducing a complementary sequence (or 'key') that disrupts the hairpin on the mRNA transcript allowing the Ribosome to bind and initiate translation.  To learn more, go [http://parts2.mit.edu/wiki/index.php/Berkeley2006-RiboregulatorsMain here].
    ''ii)''Inaccurate cell counts due to poor image processing.
 
#Evaluate the response of populations of E.Coli cells containing engineered genetic circuits (http://parts.mit.edu) to particular selective pressures using the Sortostat.
*[[Bryan Hernandez/UROP Proposal|UROP Proposal]]
 
===[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 Addressable Conjugation in Bacterial Networks]===
*Our project was to create an addressable cell-to-cell communication mechanism in e. coli.<br>
*[[IGEM:UC Berkeley/2006 | iGEM UC Berkeley]]
*[[IGEM:UC Berkeley/2006/bryans notebook|notebook]]<br>
 
===[[Sortostat]]===
The Sortostat is a microfluidic chemostat integrated with a cell sorter.  My project consists of demonstrating the sortostat's chemostat functionality, a technique rarely seen in microfluidics.
 
'''Immediate Goals'''
*Debug all engineering-related problems with sortostat.
*Demonstrate Sortostat's ability to attain chemostasis in a population of E. Coli.
*Demonstrate Sortostat's ability to sort two phenotypically different populations of E. Coli.  In this case, I will be using a mixture of E. Coli expressing CFP and YFP.
*Demostrate Sortostat's ability to select for an arbitrary population of cells that are phenotypically different from others and maintain this population at a steady state.
*[[Bryan Hernandez/UROP Proposal|UROP Proposal]]
*[http://www.midgard.liu.se/~b00perst/chemostat.pdf Chemostat Theory]
*[[Sortostat/Experiments]]
*[[Sortostat/Growth Tests]]
 
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[[Bryan Hernandez/20.109|20.109]]<br>
[[IGEM:UC Berkeley/2006/bryans -80 stocks|-80 stocks]]<br>
[[media:oligos.xls|oligos]]
 
contact me at bryanh (AT) mit
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Latest revision as of 07:48, 18 May 2010

For information on me, see The Bryan Hernandez Game.

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