Bryan Hernandez: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
 
(94 intermediate revisions by 3 users not shown)
Line 1: Line 1:
== '''Bio''' ==
For information on me, see [http://thebryanhernandezgame.wordpress.com/ The Bryan Hernandez Game].
*I am currently a UROP in the Endy Lab working with [[Jason Kelly]].
<!--
*Class of 2009; Course 18 and BE.
== Bio ==
*NorCal=home


*I am currently an undergraduate researcher in the [[Endy Lab]] at MIT.  I am currently building and characterizing orthogonal riboregulators.  Learn more about riboregulators and the work I have done [http://parts2.mit.edu/wiki/index.php/Berkeley2006-RiboregulatorsMain here]
*MIT Class of 2009; Majoring in [http://math.mit.edu/| Mathematics] and [http://web.mit.edu/be/index.htm| Biological Engineering].


== Projects ==
== Projects ==
#[[Sortostat]]
#*We are currently using strains of E.Coli expressing CFP (Cyan Fluorescent Protein) and YFP (Yellow Fluorescent Protein) as these are easily distinguished between visually thereby aiding in our descriminitive assortment without relying on chemical selection.  In this way, it is less likely for cells to avoid being sorted by a genetic mutation as one might express using an antibiotic.       
#...


'''Project Goals'''
===Construction and Characterization of Riboregulators===
A Riboregulator is a post-transcriptional regulator used in bacteria.  Its mechanism for regulation involves the occlusion of the Ribosome via a hairpin on the mRNA transcript in the 'off' state thereby preventing (or 'locking') translation of the ORF.  The 'on' state is recovered by introducing a complementary sequence (or 'key') that disrupts the hairpin on the mRNA transcript allowing the Ribosome to bind and initiate translation.  To learn more, go [http://parts2.mit.edu/wiki/index.php/Berkeley2006-RiboregulatorsMain here].


[[Sortostat]]
*[[Bryan Hernandez/UROP Proposal|UROP Proposal]]


#Debug a microfluidic chemostat ([[Sortostat]]) to improve the time-varying specific selection of cell populations.  
===[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 Addressable Conjugation in Bacterial Networks]===
#*Current problems include:  i) Cell Death after 3-4 days (presumably due to oxygen depletion,) and ii) Inaccurate cell counts due to poor image processing.
*Our project was to create an addressable cell-to-cell communication mechanism in e. coli.<br>
#Evaluate the response of populations of E.Coli cells containing engineered genetic circuits (http://parts.mit.edu) to particular selective pressures using the Sortostat.
*[[IGEM:UC Berkeley/2006 | iGEM UC Berkeley]]
*[[IGEM:UC Berkeley/2006/bryans notebook|notebook]]<br>
 
===[[Sortostat]]===
The Sortostat is a microfluidic chemostat integrated with a cell sorter. My project consists of demonstrating the sortostat's chemostat functionality, a technique rarely seen in microfluidics.
 
'''Immediate Goals'''
*Debug all engineering-related problems with sortostat.
*Demonstrate Sortostat's ability to attain chemostasis in a population of E. Coli.
*Demonstrate Sortostat's ability to sort two phenotypically different populations of E. Coli.  In this case, I will be using a mixture of E. Coli expressing CFP and YFP.
*Demostrate Sortostat's ability to select for an arbitrary population of cells that are phenotypically different from others and maintain this population at a steady state.
*[[Bryan Hernandez/UROP Proposal|UROP Proposal]]
*[http://www.midgard.liu.se/~b00perst/chemostat.pdf Chemostat Theory]
*[[Sortostat/Experiments]]
*[[Sortostat/Growth Tests]]
 
-----------
 
[[Bryan Hernandez/20.109|20.109]]<br>
[[IGEM:UC Berkeley/2006/bryans -80 stocks|-80 stocks]]<br>
[[media:oligos.xls|oligos]]
 
contact me at bryanh (AT) mit
-->

Latest revision as of 07:48, 18 May 2010

For information on me, see The Bryan Hernandez Game.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Bryan_Hernandez&oldid=415618"