Bryan Hernandez

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(Construction and Characterization of Riboregulators)
([http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006| Addressable Conjugation in Bacterial Networks])
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*[[Bryan Hernandez/UROP Proposal|UROP Proposal]]
*[[Bryan Hernandez/UROP Proposal|UROP Proposal]]
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===[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006| Addressable Conjugation in Bacterial Networks]===
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===[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 Addressable Conjugation in Bacterial Networks]===
[[IGEM:UC Berkeley/2006 | iGEM UC Berkeley]]
[[IGEM:UC Berkeley/2006 | iGEM UC Berkeley]]
*Our project was to create an addressable cell-to-cell communication mechanism in e. coli.<br>
*Our project was to create an addressable cell-to-cell communication mechanism in e. coli.<br>

Revision as of 03:08, 23 November 2006

Contents

Projects

Construction and Characterization of Riboregulators

A Riboregulator is a post-transcriptional regulator used in bacteria. Its mechanism for regulation involves the occlusion of the Ribosome via a hairpin on the mRNA transcript in the 'off' state thereby preventing (or 'locking') translation of the ORF. The 'on' state is recovered by introducing a complementary sequence (or 'key') that disrupts the hairpin on the mRNA transcript allowing the Ribosome to bind and initiate translation. To learn more, go here.

Addressable Conjugation in Bacterial Networks

iGEM UC Berkeley

  • Our project was to create an addressable cell-to-cell communication mechanism in e. coli.

notebook

Sortostat

The Sortostat is a microfluidic chemostat integrated with a cell sorter. My project consists of demonstrating the sortostat's chemostat functionality, a technique rarely seen in microfluidics.

Immediate Goals

  • Debug all engineering-related problems with sortostat.
  • Demonstrate Sortostat's ability to attain chemostasis in a population of E. Coli.
  • Demonstrate Sortostat's ability to sort two phenotypically different populations of E. Coli. In this case, I will be using a mixture of E. Coli expressing CFP and YFP.
  • Demostrate Sortostat's ability to select for an arbitrary population of cells that are phenotypically different from others and maintain this population at a steady state.
  • UROP Proposal
  • Chemostat Theory
  • Sortostat/Experiments
  • Sortostat/Growth Tests

-80 stocks
oligos

Bio

  • I am currently an undergraduate researcher in the Endy Lab at MIT. I am currently building and characterizing orthogonal riboregulators. Learn more about riboregulators and the work I have done here
  • MIT Class of 2009; Majoring in Mathematics and Biological Engineering.
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