Bryan Hernandez: Difference between revisions
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== Projects == | == Projects == | ||
===[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006| Addressable Conjugation in Bacterial Networks]=== | ===[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006| Addressable Conjugation in Bacterial Networks]=== | ||
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===[[Sortostat]]=== | ===[[Sortostat]]=== | ||
The Sortostat is a microfluidic chemostat integrated with a cell sorter. My project consists of demonstrating the sortostat's chemostat functionality, a technique rarely seen in microfluidics. | The Sortostat is a microfluidic chemostat integrated with a cell sorter. My project consists of demonstrating the sortostat's chemostat functionality, a technique rarely seen in microfluidics. | ||
'''Immediate Goals''' | |||
*Debug all engineering-related problems with sortostat. | |||
*Demonstrate Sortostat's ability to attain chemostasis in a population of E. Coli. | |||
*Demonstrate Sortostat's ability to sort two phenotypically different populations of E. Coli. In this case, I will be using a mixture of E. Coli expressing CFP and YFP. | |||
*Demostrate Sortostat's ability to select for an arbitrary population of cells that are phenotypically different from others and maintain this population at a steady state. | |||
*[[Bryan Hernandez/UROP Proposal|UROP Proposal]] | |||
*[http://www.midgard.liu.se/~b00perst/chemostat.pdf Chemostat Theory] | *[http://www.midgard.liu.se/~b00perst/chemostat.pdf Chemostat Theory] | ||
*[[Sortostat/Experiments]] | *[[Sortostat/Experiments]] | ||
*[[Sortostat/Growth Tests]] | *[[Sortostat/Growth Tests]] | ||
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[[IGEM:UC Berkeley/2006/bryans -80 stocks|-80 stocks]]<br> | |||
[[media:oligos.xls|oligos]] | |||
== Bio == | == Bio == |
Revision as of 23:51, 22 November 2006
Projects
Addressable Conjugation in Bacterial Networks
- Our project was to create an addressable cell-to-cell communication mechanism in e. coli.
Sortostat
The Sortostat is a microfluidic chemostat integrated with a cell sorter. My project consists of demonstrating the sortostat's chemostat functionality, a technique rarely seen in microfluidics.
Immediate Goals
- Debug all engineering-related problems with sortostat.
- Demonstrate Sortostat's ability to attain chemostasis in a population of E. Coli.
- Demonstrate Sortostat's ability to sort two phenotypically different populations of E. Coli. In this case, I will be using a mixture of E. Coli expressing CFP and YFP.
- Demostrate Sortostat's ability to select for an arbitrary population of cells that are phenotypically different from others and maintain this population at a steady state.
- UROP Proposal
- Chemostat Theory
- Sortostat/Experiments
- Sortostat/Growth Tests
Bio
- I am currently an undergraduate researcher in the Endy Lab at MIT. I am currently building and characterizing orthogonal riboregulators. Learn more about riboregulators and the work I have done here
- MIT Class of 2009; Majoring in Mathematics and Biological Engineering.