Buckner Lab:Notebook/Summer 2010/2010/05/18: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Bryce Bond (talk | contribs) (Autocreate 2010/05/18 Entry for Buckner_Lab:Notebook/Summer_2010) |
Bryce Bond (talk | contribs) |
||
(4 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Plasmids, Stocks</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Plasmid Isolation and Liquid Media== | ||
* | Objectives: | ||
*Analyze bacterial cultures inoculated 5/17 | |||
*Prepare LB Broth medium for the Synthetic Biology Lab | |||
*Make a ampicillin stock for the SynBio Lab. | |||
*Make overnight cultures | |||
====Bacteria Results==== | |||
{| border="1" | |||
|+ fas1b 2, KER 1D, Kn1 Recombined Plasmids in E.coli JM109 | |||
! Bacterial Strain !! Growth (+/-) !! Description | |||
|- | |||
! JM 109 25μL | |||
| + || Restreak (MC) | |||
|- | |||
! JM 109 100μL | |||
| + | |||
| Good Growth (MC) | |||
|- | |||
! Kn1 25μL | |||
| + || Sm iso colonies | |||
|- | |||
! Kn1 100μL | |||
| + || MC to isolate | |||
|- | |||
! KER 1D 25μL | |||
| + || Sm iso colonies | |||
|- | |||
! KER 1D 100μL | |||
| + || MC to isolate | |||
|- | |||
! fas1b 2 25μL | |||
| + || iso colonies | |||
|- | |||
! fas1b 2 100μL | |||
| + || MC to isolate | |||
|} | |||
MC= "multiple colonies present on agar"<br> | |||
==LB Broth and Ampicillin Stock== | |||
-Made 300mL of LB broth media. Autoclaved at 9:47am <br> | |||
-Poured broth into 6, 50mL portions. Stored in SynBio Lab at room temp <br> | |||
-Started to make 10mL Amp (50mg/mL concentration) but spilled some during sterilization. Ended making 6mL of Amp stock and separating into 7 x's 700μL portions. Placed sterilized Amp in bullet box in Buckner freezer. | |||
Back to [[Buckner Lab]]<br> | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 10:10, 18 May 2010
Plasmids, Stocks | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||
Plasmid Isolation and Liquid MediaObjectives:
Bacteria Results
MC= "multiple colonies present on agar" LB Broth and Ampicillin Stock-Made 300mL of LB broth media. Autoclaved at 9:47am
|