Buckner Lab:Notebook/Summer 2010/2010/05/18: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Bryce Bond (talk | contribs) No edit summary |
Bryce Bond (talk | contribs) No edit summary |
||
Line 15: | Line 15: | ||
====Bacteria Results==== | ====Bacteria Results==== | ||
{| border="1" | {| border="1" | ||
|+ fas1b 2, KER 1D, Kn1 Recombined Plasmids in | |+ fas1b 2, KER 1D, Kn1 Recombined Plasmids in E.coli JM109 | ||
! Bacterial Strain !! Growth (+/-) !! Description | ! Bacterial Strain !! Growth (+/-) !! Description | ||
|- | |- | ||
Line 43: | Line 43: | ||
| + || MC to isolate | | + || MC to isolate | ||
|} | |} | ||
MC= "multiple colonies present on agar" | MC= "multiple colonies present on agar"<br> | ||
==LB Broth and Ampicillin Stock== | |||
-Made 300mL of LB broth media. Autoclaved at 9:47am <br> | |||
-Poured broth into 6, 50mL portions. Stored in SynBio Lab at room temp <br> | |||
-Started to make 10mL Amp (50mg/mL concentration) but spilled some during sterilization. Ended making 6mL of Amp stock and separating into 7 x's 700μL portions. Placed sterilized Amp in bullet box in Buckner freezer. | |||
Revision as of 10:03, 18 May 2010
Plasmids, Stocks | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||
Plasmid Isolation and Liquid MediaObjectives:
Bacteria Results
MC= "multiple colonies present on agar" LB Broth and Ampicillin Stock-Made 300mL of LB broth media. Autoclaved at 9:47am
|