Buckner Lab:Notebook/Summer 2010/2010/05/19
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| - | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | + | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">DNA Purification and Gel Electrophoresis</span> |
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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| - | == | + | ==Plasmid Isolation== |
| - | * | + | * Used QIAprep isolation kit from Qiagen to isolate plasmids. |
| + | * Performed EcoR1 digestion to isolated DNA for ~2.25 hrs | ||
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| + | ==Pouring and Running Gel== | ||
| + | * Made a 0.8% agarose gel with ethidium bromide | ||
| + | * Ran the gel for 1.5 hrs in TAE buffer at 60V. | ||
| + | * Photographed and annotated gels | ||
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| + | -Didn't allow the gel to run long enough. <br> | ||
| + | -Need to add all of the DNA from the prep tube when taking a "look" after an EcoRI digestion | ||
Revision as of 14:55, 21 May 2010
 DNA Purification and Gel Electrophoresis
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Plasmid Isolation
Pouring and Running Gel
-Didn't allow the gel to run long enough.
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