Burkart:Competent Cell: Difference between revisions
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==Materials and Equipments== | ==Materials and Equipments== | ||
===RF1 buffer=== | |||
<p> RbCl 100mM (12g/L)</p> | |||
<p> MnCl<sub>2</sub>·4H<sub>2</sub>O 50mM (9.9g/L)</p> | |||
<p> Potassium Acetate 30mM (2.9g/L)</p> | |||
<p> CaCl<sub>2</sub>·2H<sub>2</sub>O 10mM (1.5g/L)</p> | |||
<p> Glycerol 15%(w/v)</p> | |||
<p> Adjust pH to 5.8 with acetic acid. Sterilize.</p> | |||
==Protocol== | ==Protocol== | ||
==Note== | ==Note== | ||
Revision as of 12:37, 24 April 2009
RbCl Competent Cell Preparation
Introduction
Materials and Equipments
RF1 buffer
RbCl 100mM (12g/L)
MnCl2·4H2O 50mM (9.9g/L)
Potassium Acetate 30mM (2.9g/L)
CaCl2·2H2O 10mM (1.5g/L)
Glycerol 15%(w/v)
Adjust pH to 5.8 with acetic acid. Sterilize.
