Bxb1: Difference between revisions
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[http://www.ncbi.nlm.nih.gov/pubmed/11123671?ordinalpos=14&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [1] | [http://www.ncbi.nlm.nih.gov/pubmed/11123671?ordinalpos=14&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [1] | ||
[http://www.ncbi.nlm.nih.gov/pubmed/16719562?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [2] | ''Genome organization and characterization of mycobacteriophage Bxb1.'', mediavilla et al., Mol microbiol, 2000. PMID 1123671. | ||
[http://www.ncbi.nlm.nih.gov/pubmed/16629393?ordinalpos=8&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [ | |||
[http://www.ncbi.nlm.nih.gov/pubmed/16719562?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [4][2] ''Control of phage Bxb1 excision by a novel recombination directionality factor.'', Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562. | |||
[http://www.ncbi.nlm.nih.gov/pubmed/16629393?ordinalpos=8&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [5] ''Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells.'', Russel JP et al., Biotechniques, 2006. PMID:16629393],], ]. |
Revision as of 17:37, 21 January 2009
Bxb1 bacteriophage was isolated in the Bronx, NY [1]. It infects Mycobacterium Smegmatis. Its genome is around 50.000 bp and code for 86 proteins [1]. Bxb1 integrase catalyze integration of the phage genome into the GroEL1 gene of M.segmentis, which contains the attB site [2]. Minimal attB, attP,[2],[3] attL and attR [4] have been characterized. Excisionase has been identified, is relatively big (28kDa) for a RDF, and is efficient both in vivo and in vitro [4]. In vitro recombination is very efficient ranging a 100% efficiency in 1hour for integration [2] and for excision [4]. Interestingly the excisionase subunit does not bind DNA and acts stoechiometrically on Bxb1 integrase [4]. The enzyme does not require supercoiled DNA, and can also works with linear products [2]. The enzyme also works well in human, rat, and mouse cells [5, 6].
Uniprot entry:
Bxb1 integrase Bxb1 excisionase
- att sites:
From
attP:
attB:
attL:
attR:
References:
[1 Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671.
[4[2] Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562.
[5 Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393],], ].