Bxb1: Difference between revisions
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[http://www.ncbi.nlm.nih.gov/pubmed/16719562?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [4]] ''Control of phage Bxb1 excision by a novel recombination directionality factor.'', Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562. | [http://www.ncbi.nlm.nih.gov/pubmed/16719562?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [4]] ''Control of phage Bxb1 excision by a novel recombination directionality factor.'', Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562. | ||
[http://www.ncbi.nlm.nih.gov/pubmed/16629393?ordinalpos=8&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [5]] ''Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells.'', Russel JP et al., Biotechniques, 2006. PMID:16629393 | [http://www.ncbi.nlm.nih.gov/pubmed/16629393?ordinalpos=8&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [5]] ''Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells.'', Russel JP et al., Biotechniques, 2006. PMID:16629393 | ||
[http://www.ncbi.nlm.nih.gov/pubmed/16699779?ordinalpos=7&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [6]] ''A diversity of serine phage integrases mediate site-specific recombination in mammalian cells.'' Keravala A, Groth AC, Jarrahian S, Thyagarajan B, Hoyt JJ, Kirby PJ, Calos MP. Mol Genet Genomics, 2006 PMID: 16699779 | [http://www.ncbi.nlm.nih.gov/pubmed/16699779?ordinalpos=7&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum [6]] ''A diversity of serine phage integrases mediate site-specific recombination in mammalian cells.'' Keravala A, Groth AC, Jarrahian S, Thyagarajan B, Hoyt JJ, Kirby PJ, Calos MP. Mol Genet Genomics, 2006 PMID: 16699779 |
Latest revision as of 17:53, 21 January 2009
Bxb1 bacteriophage was isolated in the Bronx, NY [1]. It infects Mycobacterium Smegmatis. Its genome is around 50.000 bp and code for 86 proteins [1]. Bxb1 integrase catalyze integration of the phage genome into the GroEL1 gene of M.segmentis, which contains the attB site [2]. Minimal attB, attP,[2],[3] attL and attR [4] have been characterized. Excisionase has been identified, is relatively big (28kDa) for a RDF, and is efficient both in vivo and in vitro [4]. In vitro recombination is very efficient ranging a 100% efficiency in 1hour for integration [2] and for excision [4]. Interestingly the excisionase subunit does not bind DNA and acts stoechiometrically on Bxb1 integrase [4]. The enzyme does not require supercoiled DNA, and can also works with linear products [2]. The enzyme also works well in human, rat, and mouse cells [5, 6].
Uniprot entry:
Bxb1 integrase Bxb1 excisionase
- att sites:
From
attP:
attB:
attL:
attR:
References:
[1] Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671.
[2] Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene. Kim AI, Ghosh P, Aaron MA, Bibb LA, Jain S, Hatfull GF. Mol Microbiol. 2003
[3] The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Ghosh P, Kim AI, Hatfull GF. Mol Cell. 2003 PMID:14636570
[4] Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562.
[5] Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393
[6] A diversity of serine phage integrases mediate site-specific recombination in mammalian cells. Keravala A, Groth AC, Jarrahian S, Thyagarajan B, Hoyt JJ, Kirby PJ, Calos MP. Mol Genet Genomics, 2006 PMID: 16699779