CAPS
From OpenWetWare
(Difference between revisions)
(Created page. Has to ask what this was, and decided to share the answer) |
m (Forgot some things,.) |
||
| Line 1: | Line 1: | ||
==Purpose== | ==Purpose== | ||
| - | CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots | + | CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol. |
==Recipe== | ==Recipe== | ||
| Line 7: | Line 7: | ||
To make 5L of 1X CAPS, | To make 5L of 1X CAPS, | ||
# Combine the following: | # Combine the following: | ||
| - | #* 11. | + | #* 11.066g CAPS |
#* 4.5L 10% Methanol | #* 4.5L 10% Methanol | ||
# Adjust pH to 7.4 with around 5mL concentrated HCl | # Adjust pH to 7.4 with around 5mL concentrated HCl | ||
Revision as of 19:46, 21 April 2009
Purpose
CAPS stands for 3-cyclohexylamino-1-propanesulfonic acid and is used in the protocol for heme staining of polyacrylamide gel blots. The powder has a formula weight of 221.32g/mol.
Recipe
1X CAPS is 10mM, pH 11
To make 5L of 1X CAPS,
- Combine the following:
- 11.066g CAPS
- 4.5L 10% Methanol
- Adjust pH to 7.4 with around 5mL concentrated HCl
- Add 10% Methanol to 5L and
