CH391L/S12/Artemisinic Acid Engineering

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Artemisia is a genus that encompass a broad range of plants commonly known as sagebrush, sagewort, and wormwood. The family, which derives its name from the either from the Greek goddess Artemis or Artemisia, the wife of Mausolus, includes many famous plants. <i>Artemisia absinthium</i> is used in the production of Absinthe, and Shakespeare employed wormwood as a cure for the love potion in Midsummer's Night Dream. One species of Artemisia, known as <i>Artemsiai annua</i>, has been used for thousands of years in traditional Chinese medicines to cure headaches.
Artemisia is a genus that encompass a broad range of plants commonly known as sagebrush, sagewort, and wormwood. The family, which derives its name from the either from the Greek goddess Artemis or Artemisia, the wife of Mausolus, includes many famous plants. <i>Artemisia absinthium</i> is used in the production of Absinthe, and Shakespeare employed wormwood as a cure for the love potion in Midsummer's Night Dream. One species of Artemisia, known as <i>Artemsiai annua</i>, has been used for thousands of years in traditional Chinese medicines to cure headaches.
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[[Image:ArtemisnicAcidPathway.jpg‎|thumb|right|Ro et al.'s procedure for converting carbohydrates into artemisinic acid in <i>S. cerevisiae</i>. <cite>Giordano-Santini2011</cite>.]]
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[[Image:Artemisia_Annua.jpg‎|thumb|left|<i>Artemisia annua</i>, a common wormwood found in the temperate province of China, Hubei. Image from Wikipedia Commons.]]
This last species is of particular interest to synthetic biologists, due to its ability to produce  
This last species is of particular interest to synthetic biologists, due to its ability to produce  
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[[Image:ArtemisnicAcidPathway.jpg‎|thumb|right|Ro et al.'s procedure for converting carbohydrates into artemisinic acid in <i>S. cerevisiae</i>. <cite>Giordano-Santini2011</cite>.]]
[[Image:ArtemisnicAcidPathway.jpg‎|thumb|right|Ro et al.'s procedure for converting carbohydrates into artemisinic acid in <i>S. cerevisiae</i>. <cite>Giordano-Santini2011</cite>.]]
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==Types of Selectable Markers==
 
==Production Procedure==
==Production Procedure==

Revision as of 16:08, 8 April 2012


  • NOTE* Page is still under construction. As long as this note appears, assume information presented on this page is incomplete and unreliable.

Contents

Introduction

Artemisia is a genus that encompass a broad range of plants commonly known as sagebrush, sagewort, and wormwood. The family, which derives its name from the either from the Greek goddess Artemis or Artemisia, the wife of Mausolus, includes many famous plants. Artemisia absinthium is used in the production of Absinthe, and Shakespeare employed wormwood as a cure for the love potion in Midsummer's Night Dream. One species of Artemisia, known as Artemsiai annua, has been used for thousands of years in traditional Chinese medicines to cure headaches.

Artemisia annua, a common wormwood found in the temperate province of China, Hubei. Image from Wikipedia Commons.
Artemisia annua, a common wormwood found in the temperate province of China, Hubei. Image from Wikipedia Commons.

This last species is of particular interest to synthetic biologists, due to its ability to produce

http://www.ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID=35608

Ro et al.'s procedure for converting carbohydrates into artemisinic acid in S. cerevisiae. [1].
Ro et al.'s procedure for converting carbohydrates into artemisinic acid in S. cerevisiae. [1].

Production Procedure

In a famous paper released by Ro et al., Saccharomyces cerevisiae was engineered to produced artemisinic acid from simple sugars.

Previous iGEM Examples

There has been no incidence of previous iGEM teams investigating Artemisinin or any of its precursors. Many iGEM teams have referenced Ro et al., but only as an example of pathway engineering, or in order to discuss ethics and synthetic biology's potential to positively benefit society. No parts in the parts registry deals with Artemisinin either. Only one [set of parts] mentions a protein, Granulysin, which can potentially be used to fight Malaria.

http://en.wikipedia.org/wiki/Artemisinin

References

  1. Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, Ho KA, Eachus RA, Ham TS, Kirby J, Chang MC, Withers ST, Shiba Y, Sarpong R, and Keasling JD. . pmid:16612385. PubMed HubMed [Ro2006]
  2. Chang MC, Eachus RA, Trieu W, Ro DK, and Keasling JD. . pmid:17438551. PubMed HubMed [Chang2007]
All Medline abstracts: PubMed HubMed
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