CH391L/S12/Assignments

In reverse chronological order...

For Monday, February 20, 2012

• Comment on or add to old topics.

New Topics: Chassis and Backbones

• Host organisms
• Artificial chromosomes and plasmids - replication origins
• Selectable genetic markers + toxin/anti-toxins -Brian Renda
• Counter-selectable genetic markers - Erik Quandt

For Monday, February 13, 2012

• Comment on or add to old topics.

New Topics: Optimizing Parts and their Interactions

• Metabolic engineering
• Deep-sequencing of randomized pools
• Directed evolution
• CAD systems for synthetic biology

For Monday, February 6, 2012

• Add a link to your user page to the CH391L Users Page.
• Add something to your user page.
• Comment on or add to old topics.

New Topics: Assembling Biological Parts

• Restriction enzyme cloning & BioBricks/BglBricks assembly - Ben Slater
• Gene/genome synthesis/Gibson assembly - Joe Hanson
• Advanced PCR techniques - Yi Kou

For Monday, January 30, 2012

• Register to use the OpenWetWare Wiki.
• Learn how to edit pages.
• Play around in the WIki sandbox.
• Leave at least one comment on the Talk page or make at least one addition to Introduction to Synthetic Biology
• Read the review article:
  1. Andrianantoandro E, Basu S, Karig DK, and Weiss R. Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol. 2006;2:2006.0028. DOI:10.1038/msb4100073 |

New Topics: Finding Biological Parts

• iGEM registry - Midhat Patel
• bioprospecting / metagenomics - David Truong
• directed evolution / in vitro selection - Adam Meyer
• ancestral protein construction - Jared Ellefson