CH391L/S12/Assignments

In reverse chronological order...

Yet to sign up for first presentation:

• Jeremy McLain
• Peter Otoupal

Other topic ideas

• Directed evolution (plasmid-based in vivo evolution of thermostability, altered substrate specificity, etc). - EMPTY
  • Possible reference [1]

1. Gupta RD, Goldsmith M, Ashani Y, Simo Y, Mullokandov G, Bar H, Ben-David M, Leader H, Margalit R, Silman I, Sussman JL, and Tawfik DS. Directed evolution of hydrolases for prevention of G-type nerve agent intoxication. Nat Chem Biol. 2011 Feb;7(2):120-5. DOI:10.1038/nchembio.510 |

• CAD systems for synthetic biology - EMPTY

For Monday, February 27, 2012

• Comment on or add to old topics.

New Topics: Basic Gene Construction

• Transcriptional promoters - Jon Laurent
• Transcription termination - Logan Bachman
• Translation. Ribosome binding sites and codon optimization - Jeremy McLain

For Monday, February 20, 2012

• Comment on or add to old topics.

New Topics: Chassis and Backbones

• Artificial chromosomes and plasmids - replication origins - EMPTY
• Selectable genetic markers (Ab resistance, etc.) - EMPTY
• Toxin/anti-toxins, plasmid addiction systems - Brian Renda
• Counter-selectable genetic markers - Erik Quandt

For Monday, February 13, 2012

• Comment on or add to old topics.

New Topics: Optimizing Parts and their Interactions

• Gene/genome synthesis/Gibson assembly - Joe Hanson
• Host organisms (general properties of common ones, new ones that are being developed) - Michael Hammerling
• Metabolic engineering - Razan Alnahhas

For Monday, February 6, 2012

• Add a link to your user page to the CH391L Users Page.
• Add something to your user page.
• Comment on or add to old topics.

New Topics: Assembling Biological Parts

• Restriction enzyme cloning & BioBricks/BglBricks assembly - Ben Slater
• Advanced PCR techniques - Yi Kou

For Monday, January 30, 2012

• Register to use the OpenWetWare Wiki.
• Learn how to edit pages.
• Play around in the WIki sandbox.
• Leave at least one comment on the Talk page or make at least one addition to Introduction to Synthetic Biology
• Read the review article:
  2. Andrianantoandro E, Basu S, Karig DK, and Weiss R. Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol. 2006;2:2006.0028. DOI:10.1038/msb4100073 |

New Topics: Finding Biological Parts

• iGEM registry - Midhat Patel
• bioprospecting / metagenomics - David Truong
• directed evolution / in vitro selection - Adam Meyer
• ancestral protein construction - Jared Ellefson