CH391L/S12/In vitro Selection

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===Library Generation===
===Library Generation===
-
 
+
=====Randomized Oligodeoxynucleotides=====
 +
=====Mutagenic PCR=====
 +
=====Gene Shuffling=====
 +
=====Neutral Drift=====
===Confinement of Function===
===Confinement of Function===
-
 
+
=====Binding=====
 +
=====Cis-action=====
 +
=====Cellularization=====
 +
=====''In vitro'' Compartmentalization=====
===Increased Representation===
===Increased Representation===
-
 
+
=====Binding=====
 +
=====Protection=====
 +
=====Selective Amplification=====
 +
=====FACS=====
==History==
==History==
-
===Aptamers===
+
===Nucleic Acids===
-
===Ribozymes===
+
=====Aptamers=====
-
===DNA Modifying Proteins===
+
Ellington 1990
-
===Other Enzymatic Functions===
+
=====Ribozymes=====
 +
Bartel 1993
 +
===Proteins===
 +
=====Antibodies=====
 +
Accepting suggestions
 +
=====DNA Modifying Proteins=====
 +
Tawfik 1998
 +
=====Other Enzymatic Functions=====
 +
Griffiths 2003
===Self-Replication===
===Self-Replication===
-
 
+
Ghadessy 2001
==References==
==References==

Revision as of 00:45, 28 January 2012

Contents

Overview of in vitro selection

Library Generation

Randomized Oligodeoxynucleotides
Mutagenic PCR
Gene Shuffling
Neutral Drift

Confinement of Function

Binding
Cis-action
Cellularization
In vitro Compartmentalization

Increased Representation

Binding
Protection
Selective Amplification
FACS

History

Nucleic Acids

Aptamers

Ellington 1990

Ribozymes

Bartel 1993

Proteins

Antibodies

Accepting suggestions

DNA Modifying Proteins

Tawfik 1998

Other Enzymatic Functions

Griffiths 2003

Self-Replication

Ghadessy 2001


References

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