CH391L/S12/Origins of Replication: Difference between revisions
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[[Category:CH391L_S12]] | [[Category:CH391L_S12]] | ||
==Origins of Replication== | ==Origins of Replication== | ||
===Modes of replication=== | ===Modes of replication=== | ||
====Rolling circle==== | ====Rolling circle==== | ||
| Line 9: | Line 9: | ||
===Incompatibility Groups=== | ===Incompatibility Groups=== | ||
*ColE1 | *ColE1: pUC, pMB1, pBR322, pGEM, pET, pUC, pQE, pMAL, pGEX | ||
*p15A | *p15A: pBad, pACYC | ||
*pSC101 | *pSC101 | ||
*CloDF13 | |||
*ColA | |||
*RSF1030 | |||
===Copy Number=== | ===Copy Number=== | ||
*ColE1: | *ColE1: 20-700 copies | ||
*pUC: 500-700 copies | **pUC: 500-700 copies | ||
*pBR322: ~ | **pBR322: ~20 copies | ||
*pSC101: ~5 copies | *pSC101: ~5 copies | ||
*p15A: 10-12 copies | *p15A: 10-12 copies | ||
| Line 23: | Line 26: | ||
*R6K: | *R6K: | ||
*F1: ~1 copy | *F1: ~1 copy | ||
*CloDF13: 20-40 | |||
*ColA: 20-40 | |||
*RSF1030: >100 | |||
===Control of Initiation/copy number=== | ===Control of Initiation/copy number=== | ||
*ColE1: Does not require plasmid encoded initiator protein, does require DnaA binding. 1kb. Includes (1) region promoting the synthesis of RNA II, which is the primer for the leading strand (2) sequences that promote stable hybridization of RNA II to DNA (3) sequences that promote RNAse digestion of RNA II, yielding primer for leading strand. RNA I is also transcribed, and sequesters RNA II, so it is unavailable for RNAse H. | *ColE1: Does not require plasmid encoded initiator protein, does require DnaA binding. 1kb. Includes (1) region promoting the synthesis of RNA II, which is the primer for the leading strand (2) sequences that promote stable hybridization of RNA II to DNA (3) sequences that promote RNAse digestion of RNA II, yielding primer for leading strand. RNA I is also transcribed, and sequesters RNA II, so it is unavailable for RNAse H. | ||
*p15A: similar to ColE1 but different RNAs | *p15A: similar to ColE1 but different RNAs | ||
*pSC101: iterons | *pSC101: iterons | ||
Revision as of 19:51, 29 February 2012
Origins of Replication
Modes of replication
Rolling circle
Strand displacement
Theta
Incompatibility Groups
- ColE1: pUC, pMB1, pBR322, pGEM, pET, pUC, pQE, pMAL, pGEX
- p15A: pBad, pACYC
- pSC101
- CloDF13
- ColA
- RSF1030
Copy Number
- ColE1: 20-700 copies
- pUC: 500-700 copies
- pBR322: ~20 copies
- pSC101: ~5 copies
- p15A: 10-12 copies
- R1:
- RK2:
- R6K:
- F1: ~1 copy
- CloDF13: 20-40
- ColA: 20-40
- RSF1030: >100
Control of Initiation/copy number
- ColE1: Does not require plasmid encoded initiator protein, does require DnaA binding. 1kb. Includes (1) region promoting the synthesis of RNA II, which is the primer for the leading strand (2) sequences that promote stable hybridization of RNA II to DNA (3) sequences that promote RNAse digestion of RNA II, yielding primer for leading strand. RNA I is also transcribed, and sequesters RNA II, so it is unavailable for RNAse H.
- p15A: similar to ColE1 but different RNAs
- pSC101: iterons
- R1: oriR is bound by RepA promoting initiation. No iterons. Copy number controlled by copA and copB. copA is in RNA that binds the 5’ end of RepA RNA and prevents translation. CopB blocks repA transcription.
- RK2:
- R6K:
- F1:
