CH391L/S12/PCR and advanced PCR techniques: Difference between revisions

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==What's PCR?==
==What's PCR?==
PCR stands for polymerase chain reaction, which is powerful technique used for ''in vitro'' exponential amplification of a specific DNA region (target region)that lies between two regions (primers) of known DNA sequence, resulting in a large quantity of DNA (~ micrograms of DNA).
PCR stands for polymerase chain reaction, which is powerful technique used for ''in vitro'' exponential amplification of a specific DNA region (target region)that lies between two regions (primers) of known DNA sequence, resulting in a large quantity of DNA (~ micrograms of DNA).
===Short history of PCR===
The invention of PCR has long been considered as an "Eureka" event occurred on Kary Mullis' travel to his cabin[[http://www.karymullis.com/pcr.shtml/ Eureka moment]]. However, it would not happen if the following milestones in molecular biology had not taken place:

Revision as of 20:05, 5 February 2012

What's PCR?

PCR stands for polymerase chain reaction, which is powerful technique used for in vitro exponential amplification of a specific DNA region (target region)that lies between two regions (primers) of known DNA sequence, resulting in a large quantity of DNA (~ micrograms of DNA).

Short history of PCR

The invention of PCR has long been considered as an "Eureka" event occurred on Kary Mullis' travel to his cabin[Eureka moment]. However, it would not happen if the following milestones in molecular biology had not taken place: