CH391L/S12/PCR and advanced PCR techniques: Difference between revisions
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===Short history of PCR=== | ===Short history of PCR=== | ||
The invention of PCR has long been considered as an "Eureka" event occurred on Kary Mullis' travel to his cabin[[http://www.karymullis.com/pcr.shtml/ Eureka moment]]. However, it would not happen if the following milestones in molecular biology had not taken place: | The invention of PCR has long been considered as an "Eureka" event occurred on Kary Mullis' travel to his cabin[[http://www.karymullis.com/pcr.shtml/ Eureka moment]]. However, it would not happen if the following milestones in molecular biology had not taken place: | ||
* Complementary double-stranded DNA model proposed by Watson and Crick <cite>WC DNA</cite>. | |||
Revision as of 20:11, 5 February 2012
What's PCR?
PCR stands for polymerase chain reaction, which is powerful technique used for in vitro exponential amplification of a specific DNA region (target region)that lies between two regions (primers) of known DNA sequence, resulting in a large quantity of DNA (~ micrograms of DNA).
Short history of PCR
The invention of PCR has long been considered as an "Eureka" event occurred on Kary Mullis' travel to his cabin[Eureka moment]. However, it would not happen if the following milestones in molecular biology had not taken place:
