CH391L/S12/PCR and advanced PCR techniques: Difference between revisions
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* Complementary double-stranded DNA model proposed by Watson and Crick <cite>WCDNA</cite>. | * Complementary double-stranded DNA model proposed by Watson and Crick <cite>WCDNA</cite>. | ||
* Studies on DNA replication and polymerase by Arthur Kornberg in 1950s <cite>AKDNApol</cite>. | * Studies on DNA replication and polymerase by Arthur Kornberg in 1950s <cite>AKDNApol</cite>. | ||
* [http://en.wikipedia.org/wiki/Klenow_fragment/ Klenow fragment]from ''E. coli''<cite>Kfragment</cite>. | * [http://en.wikipedia.org/wiki/Klenow_fragment/ Klenow fragment] from ''E. coli'' <cite>Kfragment</cite>. | ||
==References== | ==References== | ||
Revision as of 20:23, 5 February 2012
What's PCR?
PCR stands for polymerase chain reaction, which is powerful technique used for in vitro exponential amplification of a specific DNA region (target region)that lies between two regions (primers) of known DNA sequence, resulting in a large quantity of DNA (~ micrograms of DNA).
Short history of PCR
The invention of PCR has long been considered as an "Eureka" event occurred on Kary Mullis' travel to his cabin[Eureka moment]. However, it would not happen if the following milestones in molecular biology had not taken place:
- Complementary double-stranded DNA model proposed by Watson and Crick [1].
- Studies on DNA replication and polymerase by Arthur Kornberg in 1950s [2].
- Klenow fragment from E. coli [3].
References
- WATSON JD and CRICK FH. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953 Apr 25;171(4356):737-8. DOI:10.1038/171737a0 |
- LEHMAN IR, BESSMAN MJ, SIMMS ES, and KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. J Biol Chem. 1958 Jul;233(1):163-70.
