CH391L/S13/Assignments
For Monday, January 28th, 2013
- Register to use the OpenWetWare Wiki.
- Learn how to edit pages.
- Play around in the WIki sandbox.
- Add your name to the CH391L Users page.
- Leave at least one comment on the Talk page or make at least one addition to either Introduction to Synthetic Biology or Ethics of synthetic biology.
- Add at least one event to the "Timeline of Synthetic Biology" in the Introduction to Synthetic Biology with a link or citation.
- Read articles for discussion:
New Topics:
- Ethics of synthetic biology (Neil)
- DIY Biology Movement (John)
- BioBricks and the iGEM registry (Tyler)
For Monday, February 4th, 2013
Topic Updates
- Ethics of synthetic biology [1]
- DIY Biology[2]
- BioBricks and the iGEM registry [3]
Papers for Discussion
-
For Bioethics
-
Outlaw, hackers, victorian amateurs: diagnosing public participation in the life sciences today pdf
For DIY Biology
- Kelly JR, Rubin AJ, Davis JH, Ajo-Franklin CM, Cumbers J, Czar MJ, de Mora K, Glieberman AL, Monie DD, and Endy D. Measuring the activity of BioBrick promoters using an in vivo reference standard. J Biol Eng. 2009 Mar 20;3:4. DOI:10.1186/1754-1611-3-4 |
For BioBricks
New Topics
- CAD for Synthetic Biology (Kevin)
- DNA Assembly: From Genes to Genomes (Gabe) [4]
For Monday, February 11th, 2013
Topic Updates
- CAD for Synthetic Biology (Kevin)
- Chris Yellman and Randy Hughes will discuss the paper for DNA Assembly: From Genes to Genomes[4]
Papers for Discussion
- Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, and Smith HO. Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science. 2008 Feb 29;319(5867):1215-20. DOI:10.1126/science.1151721 |
Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. For DNA synthesis and assembly
-
Find a useful or neat tool from a past iGEM software competition using one of these links. Test drive it if possible. Be able to briefly describe what it does to class. For CAD
New Topics
- Minimal / "clean" genomes (Evan)
- Functional Nucleic Acids --Alvaro E. Rodriguez M. 21:50, 27 January 2013 (EST)
For Monday, February 18th, 2013
Topic Updates
- Minimal / "clean" genomes (Evan)
- Functional Nucleic Acids (Alvaro)
Papers for Discussion
- Pósfai G, Plunkett G 3rd, Fehér T, Frisch D, Keil GM, Umenhoffer K, Kolisnychenko V, Stahl B, Sharma SS, de Arruda M, Burland V, Harcum SW, and Blattner FR. Emergent properties of reduced-genome Escherichia coli. Science. 2006 May 19;312(5776):1044-6. DOI:10.1126/science.1126439 |
Emergent properties of reduced-genome Escherichia coli. For minimal genomes.
- Ohuchi M, Murakami H, and Suga H. The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus. Curr Opin Chem Biol. 2007 Oct;11(5):537-42. DOI:10.1016/j.cbpa.2007.08.011 |
The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus. For in vitro selection.
- Murakami H, Ohta A, Goto Y, Sako Y, and Suga H. Flexizyme as a versatile tRNA acylation catalyst and the application for translation. Nucleic Acids Symp Ser (Oxf). 2006(50):35-6. DOI:10.1093/nass/nrl018 |
Flexizyme as a versatile tRNA acylation catalyst and the application for translation. For in vitro selection.
New Topics
- Ancestral sequence reconstruction (Aurko)
- Directed protein evolution (Max)
- Bioprospecting / Metagenomics (Andre)
For Monday, February 25th, 2013
New Topics
- Biocontainment (Thomas)
- New Marker Genes (Ben)
- Optogenetics (Sid)
- TBD (Larry)
- Riboswitches (Kate)
Papers for Discussion
- Risso VA, Gavira JA, Mejia-Carmona DF, Gaucher EA, and Sanchez-Ruiz JM. Hyperstability and substrate promiscuity in laboratory resurrections of Precambrian β-lactamases. J Am Chem Soc. 2013 Feb 27;135(8):2899-902. DOI:10.1021/ja311630a |
Hyperstability and substrate promiscuity in laboratory resurrections of Precambrian β-lactamases. For Ancestral protein reconstruction.
For Monday, March 4th, 2013
- TBD (Jeremy)
- Biological material assembly and patterning - Belcher virus work (Marco)