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#Larionov1996 pmid=8552668
#Larionov1996 pmid=8552668
//Transformation-associated recombination (TAR) cloning
//Transformation-associated recombination (TAR) cloning
#Hillson2012 j5 DNA Assembly Design Automation Software [[doi: 10.1021/sb2000116]]
//j5 DNA Assembly Design Automation Software [[doi: 10.1021/sb2000116]]
[doi: 10.1021/sb2000116]]
[[doi: 10.1021/sb2000116]]

Revision as of 22:30, 21 January 2013



A large number of parts have been made by the synthetic biology community. Many can be found as part of the Registry of Standard Biological Parts. These modular genetic components are designed to be easy to assemble to facilitate the building of more complex biological devices. To learn more about the Registry and BioBricks™, see last year's wiki page.

Synthetic biology relies on the ability to make testable biological units. This means that the ability to gather, manipulate, or even create genetic material is vital for "doing" synthetic biology. The techniques employed by synthetic biologists include oligonucleotide synthesis, restriction enzyme digestion, ligase, pcr, and recombination (homology).

Oligonucleotide Synthesis

from wikipedia

Gene Synthesis

IDT Gene Blocks
invitrogen (life technologies, geneart)
OriGene (blue heron)
Epoch Life Science

Cloning Techniques

TOPO TA cloning (invitrogen)

Restriction Enzyme


in vivo techniques

  • e coli
  • s. cerevisiae


-cold fusion
-in fusion
-lambda red
-golden gate
-MoClo [1]


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  22. Angrand PO, Daigle N, van der Hoeven F, Schöler HR, and Stewart AF. . pmid:10446259. PubMed HubMed [Angrand1999]
    lambda Red recombinase

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    Gateway lambda Int

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    Transformation-associated recombination (TAR) cloning

  26. j5 DNA Assembly Design Automation Software doi: 10.1021/sb2000116 [Hillson2012]
All Medline abstracts: PubMed HubMed

doi: 10.1021/sb2000116

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