CH391L/S13/DnaAssembly
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Synthetic biology relies on the ability to make testable biological units. This means that the ability to gather, manipulate, or even create genetic material is vital for "doing" synthetic biology. The techniques employed by synthetic biologists include oligonucleotide synthesis, restriction enzyme digestion, ligase, pcr, and recombination (homology). | Synthetic biology relies on the ability to make testable biological units. This means that the ability to gather, manipulate, or even create genetic material is vital for "doing" synthetic biology. The techniques employed by synthetic biologists include oligonucleotide synthesis, restriction enzyme digestion, ligase, pcr, and recombination (homology). | ||
| - | ==Oligonucleotide Synthesis== | + | ==DNA Synthesis== |
| + | ===Oligonucleotide Synthesis=== | ||
[http://en.wikipedia.org/wiki/Oligonucleotide_synthesis from wikipedia] <br> | [http://en.wikipedia.org/wiki/Oligonucleotide_synthesis from wikipedia] <br> | ||
| - | ==Gene Synthesis== | + | ===Gene Synthesis=== |
IDT Gene Blocks <br> | IDT Gene Blocks <br> | ||
| Line 25: | Line 26: | ||
Epoch Life Science <br> | Epoch Life Science <br> | ||
| - | ==Cloning | + | ==Molecular Cloning== |
TOPO TA cloning (invitrogen) <br> | TOPO TA cloning (invitrogen) <br> | ||
Revision as of 23:44, 21 January 2013
Contents |
Introduction
A large number of parts have been made by the synthetic biology community. Many can be found as part of the Registry of Standard Biological Parts. These modular genetic components are designed to be easy to assemble to facilitate the building of more complex biological devices. To learn more about the Registry and BioBricks™, see last year's wiki page.
Synthetic biology relies on the ability to make testable biological units. This means that the ability to gather, manipulate, or even create genetic material is vital for "doing" synthetic biology. The techniques employed by synthetic biologists include oligonucleotide synthesis, restriction enzyme digestion, ligase, pcr, and recombination (homology).
DNA Synthesis
Oligonucleotide Synthesis
Gene Synthesis
IDT Gene Blocks
DNA2.0
invitrogen (life technologies, geneart)
genscript
celtek
biobasic
sigma?
GENEWIZ
biomatik
OriGene (blue heron)
Epoch Life Science
Molecular Cloning
TOPO TA cloning (invitrogen)
Restriction Enzyme
-BioBricks
-BglBricks
in vivo techniques
- e coli
- s. cerevisiae
Recombination
-homology/ligase
-cold fusion
-in fusion
-lambda red
-golden gate
-lic
-slic
-mage
-Gibson
-CPEC
-MoClo [1]
References
- Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. . pmid:21364738.
MoClo - Hughes RA, Miklos AE, and Ellington AD. . pmid:21601682.
Gene Synthesis Review - Werner S, Engler C, Weber E, Gruetzner R, and Marillonnet S. . pmid:22126803.
MoClo - Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, and Orzaez D. . pmid:21750718.
GoldenBraid - Engler C, Kandzia R, and Marillonnet S. . pmid:18985154.
GoldenGate - Quan J and Tian J. . pmid:19649325.
CPEC - Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:18218864.
- Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:19363495.
T5 exonuclease recombination - Li MZ and Elledge SJ. . pmid:17293868.
SLIC - Li MZ and Elledge SJ. . pmid:22328425.
SLIC - Sleight SC, Bartley BA, Lieviant JA, and Sauro HM. . pmid:20385581.
In-Fusion biobrick - Zhu B, Cai G, Hall EO, and Freeman GJ. . pmid:17907578.
in-fusion - Benoit RM, Wilhelm RN, Scherer-Becker D, and Ostermeier C. . pmid:16289702.
in-fusion - Geu-Flores F, Nour-Eldin HH, Nielsen MT, and Halkier BA. . pmid:17389646.
USER - Gibson DG. . pmid:19745056.
oligonucleotide assembly - Horton RM, Cai ZL, Ho SN, and Pease LR. . pmid:2357375.
SOEing - Czar MJ, Anderson JC, Bader JS, and Peccoud J. . pmid:19111926.
review - Stemmer WP, Crameri A, Ha KD, Brennan TM, and Heyneker HL. . pmid:7590320.
PCA - Aslanidis C and de Jong PJ. . pmid:2235490.
LIC - Aslanidis C, de Jong PJ, and Schmitz G. . pmid:7580902.
LIC - Li C and Evans RM. . pmid:9321675.
LIC - Angrand PO, Daigle N, van der Hoeven F, Schöler HR, and Stewart AF. . pmid:10446259.
lambda Red recombinase - Hartley JL, Temple GF, and Brasch MA. . pmid:11076863.
Gateway lambda Int - Khalil AM, Julius JA, and Bachant J. . pmid:17702758.
Gateway lambda Cre - Larionov V, Kouprina N, Graves J, Chen XN, Korenberg JR, and Resnick MA. . pmid:8552668.
Transformation-associated recombination (TAR) cloning - j5 DNA Assembly Design Automation Software doi: 10.1021/sb2000116
- Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, and Venter JC. . pmid:20488990.
genome replacement
