CH391L/S13/DnaAssembly

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Introduction

Oligonucleotide Synthesis

from wikipedia

Gene Synthesis

IDT Gene Blocks

Cloning Techniques

TOPO TA cloning (invitrogen)

Restriction Enzyme

-BioBricks
-BglBricks

in vivo techniques

• e coli
  
• s. cerevisiae
  

Recombination

-homology/ligase
-cold fusion
-in fusion
-lambda red
-golden gate
-lic
-slic
-mage
-Gibson
-CPEC
-MoClo [1]

References

1. Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. . pmid:21364738. PubMed HubMed [Weber2011]

   MoClo

2. Werner S, Engler C, Weber E, Gruetzner R, and Marillonnet S. . pmid:22126803. PubMed HubMed [Werner2012]

   MoClo

3. Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, and Orzaez D. . pmid:21750718. PubMed HubMed [SarrionPerdigones2011]

   GoldenBraid

4. Engler C, Kandzia R, and Marillonnet S. . pmid:18985154. PubMed HubMed [Engler2008]

   GoldenGate

5. Quan J and Tian J. . pmid:19649325. PubMed HubMed [Quan2009]

   CPEC

6. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, and Smith HO. . pmid:19363495. PubMed HubMed [Gibson2009]

   T5 exonuclease recombination

7. Li MZ and Elledge SJ. . pmid:17293868. PubMed HubMed [Li2007]

   SLIC

8. Li MZ and Elledge SJ. . pmid:22328425. PubMed HubMed [Li2012]

   SLIC

9. Sleight SC, Bartley BA, Lieviant JA, and Sauro HM. . pmid:20385581. PubMed HubMed [Sleight2010]

   In-Fusion biobrick

10. Zhu B, Cai G, Hall EO, and Freeman GJ. . pmid:17907578. PubMed HubMed [Zhu2007]

    in-fusion

11. Benoit RM, Wilhelm RN, Scherer-Becker D, and Ostermeier C. . pmid:16289702. PubMed HubMed [Benoit2006]

    in-fusion

12. Geu-Flores F, Nour-Eldin HH, Nielsen MT, and Halkier BA. . pmid:17389646. PubMed HubMed [GeuFlores2007]

    USER

13. Gibson DG. . pmid:19745056. PubMed HubMed [Gibson2009-2]

    oligonucleotide assembly

14. Horton RM, Cai ZL, Ho SN, and Pease LR. . pmid:2357375. PubMed HubMed [Horton2009]

    SOEing

15. Czar MJ, Anderson JC, Bader JS, and Peccoud J. . pmid:19111926. PubMed HubMed [Czar2009]

    review

16. Stemmer WP, Crameri A, Ha KD, Brennan TM, and Heyneker HL. . pmid:7590320. PubMed HubMed [Stemmer1995]

    PCA

17. Aslanidis C and de Jong PJ. . pmid:2235490. PubMed HubMed [Aslanidis1990]

    LIC

18. Aslanidis C, de Jong PJ, and Schmitz G. . pmid:7580902. PubMed HubMed [Aslanidis1994]

    LIC

19. Li C and Evans RM. . pmid:9321675. PubMed HubMed [Li1997]

    LIC

20. Angrand PO, Daigle N, van der Hoeven F, Schöler HR, and Stewart AF. . pmid:10446259. PubMed HubMed [Angrand1999]

    lambda Red recombinase

21. Hartley JL, Temple GF, and Brasch MA. . pmid:11076863. PubMed HubMed [Hartley2000]

    Gateway lambda Int

22. Khalil AM, Julius JA, and Bachant J. . pmid:17702758. PubMed HubMed [Khalil2007]

    Gateway lambda Cre