CH391L/S13/DnaAssembly

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Contents

Introduction

Oligonucleotide Synthesis

from wikipedia

Gene Synthesis

IDT Gene Blocks

Cloning Techniques

TOPO TA cloning (invitrogen)

Restriction Enzyme

-BioBricks
-BglBricks


in vivo techniques

  • e coli
  • s. cerevisiae

Recombination

-homology/ligase
-cold fusion
-in fusion
-lambda red
-golden gate
-lic
-slic
-mage
-Gibson
-CPEC
-MoClo [1]

References

Error fetching PMID 21364738:
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Error fetching PMID 19111926:
Error fetching PMID 7590320:
Error fetching PMID 2235490:
Error fetching PMID 7580902:
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Error fetching PMID 17702758:
  1. Error fetching PMID 21364738: [Weber2011]
    MoClo

  2. Error fetching PMID 22126803: [Werner2012]
    MoClo

  3. Error fetching PMID 21750718: [SarrionPerdigones2011]
    GoldenBraid

  4. Error fetching PMID 18985154: [Engler2008]
    GoldenGate

  5. Error fetching PMID 19649325: [Quan2009]
    CPEC

  6. Error fetching PMID 19363495: [Gibson2009]
    T5 exonuclease recombination

  7. Error fetching PMID 17293868: [Li2007]
    SLIC

  8. Error fetching PMID 22328425: [Li2012]
    SLIC

  9. Error fetching PMID 20385581: [Sleight2010]
    In-Fusion biobrick

  10. Error fetching PMID 17907578: [Zhu2007]
    in-fusion

  11. Error fetching PMID 16289702: [Benoit2006]
    in-fusion

  12. Error fetching PMID 17389646: [GeuFlores2007]
    USER

  13. Error fetching PMID 19745056: [Gibson2009-2]
    oligonucleotide assembly

  14. Error fetching PMID 2357375: [Horton2009]
    SOEing

  15. Error fetching PMID 19111926: [Czar2009]
    review

  16. Error fetching PMID 7590320: [Stemmer1995]
    PCA

  17. Error fetching PMID 2235490: [Aslanidis1990]
    LIC

  18. Error fetching PMID 7580902: [Aslanidis1994]
    LIC

  19. Error fetching PMID 9321675: [Li1997]
    LIC

  20. Error fetching PMID 10446259: [Angrand1999]
    lambda Red recombinase

  21. Error fetching PMID 11076863: [Hartley2000]
    Gateway lambda Int

  22. Error fetching PMID 17702758: [Khalil2007]
    Gateway lambda Cre

All Medline abstracts: PubMed HubMed
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