CH391L/S13/DnaAssembly: Difference between revisions

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-Gibson <br>
-Gibson <br>
-CPEC <br>
-CPEC <br>
-MoClo  <cite>Weber2011</cite>
== References ==
<biblio>
#Weber2011 pmid=21364738
//MoClo
</biblio>

Revision as of 02:45, 21 January 2013

Oligonucleotide Synthesis

from wikipedia

Gene Synthesis

Cloning Techniques

in vitro techniques

in vivo techniques

  • e coli
  • s. cerevisiae

Restriction Enzyme
Recombination
-homology/ligase
-cold fusion
-in fusion
-lamda red
-golden gate
-slic
-mage
-Gibson
-CPEC
-MoClo [1]

References

  1. Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. DOI:10.1371/journal.pone.0016765 | PubMed ID:21364738 | HubMed [Weber2011]

    MoClo