CH391L/S13/DnaAssembly: Difference between revisions
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//MoClo | //MoClo | ||
#SarrionPerdigones2011 pmid=21750718 | #SarrionPerdigones2011 pmid=21750718 | ||
//GoldenBraid | |||
#Engler2008 pmid=18985154 | |||
//GoldenGate | |||
#Quan2009 pmid=19649325 | |||
//CPEC | |||
</biblio> | </biblio> | ||
Revision as of 02:54, 21 January 2013
Oligonucleotide Synthesis
Gene Synthesis
Cloning Techniques
in vitro techniques
in vivo techniques
- e coli
- s. cerevisiae
Restriction Enzyme
Recombination
-homology/ligase
-cold fusion
-in fusion
-lamda red
-golden gate
-slic
-mage
-Gibson
-CPEC
-MoClo [1]
References
- Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. DOI:10.1371/journal.pone.0016765 |
MoClo
- Werner S, Engler C, Weber E, Gruetzner R, and Marillonnet S. Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system. Bioeng Bugs. 2012 Jan 1;3(1):38-43. DOI:10.4161/bbug.3.1.18223 |
MoClo
- Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, Granell A, and Orzaez D. GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules. PLoS One. 2011;6(7):e21622. DOI:10.1371/journal.pone.0021622 |
GoldenBraid
- Engler C, Kandzia R, and Marillonnet S. A one pot, one step, precision cloning method with high throughput capability. PLoS One. 2008;3(11):e3647. DOI:10.1371/journal.pone.0003647 |
GoldenGate
- Quan J and Tian J. Circular polymerase extension cloning of complex gene libraries and pathways. PLoS One. 2009 Jul 30;4(7):e6441. DOI:10.1371/journal.pone.0006441 |
CPEC
