CH391L/S13/DnaAssembly
From OpenWetWare
Oligonucleotide Synthesis
Gene Synthesis
Cloning Techniques
in vitro techniques
in vivo techniques
- e coli
- s. cerevisiae
Restriction Enzyme
Recombination
-homology/ligase
-cold fusion
-in fusion
-lamda red
-golden gate
-slic
-mage
-Gibson
-CPEC
-MoClo [1]
References
- Weber E, Engler C, Gruetzner R, Werner S, and Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. DOI:10.1371/journal.pone.0016765 |
MoClo
- Werner S, Engler C, Weber E, Gruetzner R, and Marillonnet S. Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system. Bioeng Bugs. 2012 Jan 1;3(1):38-43. DOI:10.4161/bbug.3.1.18223 |