CH391L/S13/In vitro Selection of FNAs

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Contents

Introduction

Types of Functional Nucleic Acids

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

Image:CH391L_S12_Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.

In vitro Selection of Functional Nucleic Acids

Ribozymes

Deoxyribozymes

Aptamers

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