CH391L/S13/In vitro Selection of FNAs

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==In vitro Selection of Functional Nucleic Acids==
==In vitro Selection of Functional Nucleic Acids==

Revision as of 03:53, 11 February 2013



Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.

Functional Nucleic Acids




In vitro Selection of Functional Nucleic Acids



Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.


Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.

Error fetching PMID 6297745:
Error fetching PMID 6197186:
  1. Error fetching PMID 6297745: [Cech1982]
  2. Error fetching PMID 6197186: [Altman1983]
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