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#Werner2012 pmid=22126803
#Muranaka09 pmid=19190095
//Riboswitch selection/screening using a tetA-GFP fusion marker

Revision as of 01:33, 4 March 2013



The ability to introduce exogenous DNA into an organism to alter its genetic program is one of the most crucial tools in modern biology. Early work showed that certain bacteria could acquire the traits of a related strain through the addition of heat-killed cells. Although it was not well understood at the time, the transfer of gene-encoding DNA from one strain to another facilitated this. This concept was turned into a useful tool upon the advent of bacterial plasmid transformations in the early 1970's, which allowed genes of interest to be easily inserted into E. coli. Over the years, methods have been developed to introduce exogenous genes into a wide range of useful organisms, including bacteria, yeasts, plants, and animal tissues. These methods vary enormously in efficiency however, necessitating a way to identify and isolate cells which contain the DNA of interest. This can be accomplished either by screening for successfully modified cells, or through selection.

Screening vs. Selection

Antibiotic Resistance Markers




Non-Antibiotic Markers=

Novel Marker Strategies

TetA Dual Genetic Selection


Error fetching PMID 19190095:
  1. Error fetching PMID 19190095: [Muranaka09]
    Riboswitch selection/screening using a tetA-GFP fusion marker

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