CH391L/S13/In vitro Selection of FNAs

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==In vitro Selection of Functional Nucleic Acids==
==In vitro Selection of Functional Nucleic Acids==
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[[Image:In-vitro-selection.png]]

Revision as of 02:53, 11 February 2013


Contents

Introduction

Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.


Functional Nucleic Acids

[1],[2]

Ribozymes

Deoxyribozymes

In vitro Selection of Functional Nucleic Acids

Image:In-vitro-selection.png





Extra

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

Image:CH391L_S12_Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.

  1. Kruger K, Grabowski PJ, Zaug AJ, Sands J, Gottschling DE, and Cech TR. . pmid:6297745. PubMed HubMed [Cech1982]
  2. Guerrier-Takada C, Gardiner K, Marsh T, Pace N, and Altman S. . pmid:6197186. PubMed HubMed [Altman1983]
All Medline abstracts: PubMed HubMed
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