CH391L/S13/In vitro Selection of FNAs

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 21: Line 21:
==In vitro Selection of Functional Nucleic Acids==
==In vitro Selection of Functional Nucleic Acids==
-
[[Image:In-vitro-selection.png]]
+
[[Image:In-vitro-selection.png|600px|]]

Revision as of 03:54, 11 February 2013


Contents

Introduction

Functional nucleic acids (FNAs) are RNA, DNA, or XNA(nucleic acid analogues) that perform an activity such as binding or catalyzing a reaction. FNAs are grouped into three main categories Aptamers, Ribozymes, and Deoxyribozymes that are subdivided into either natural or artificial depending on their origin; the exception being Deoxyribozymes as they have yet to be discovered in a living organism.


Functional Nucleic Acids

[1],[2]

Ribozymes

Deoxyribozymes

In vitro Selection of Functional Nucleic Acids





Extra

Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.

Image:CH391L_S12_Phosphoramidite.png

Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.

  1. Kruger K, Grabowski PJ, Zaug AJ, Sands J, Gottschling DE, and Cech TR. . pmid:6297745. PubMed HubMed [Cech1982]
  2. Guerrier-Takada C, Gardiner K, Marsh T, Pace N, and Altman S. . pmid:6197186. PubMed HubMed [Altman1983]
All Medline abstracts: PubMed HubMed
Personal tools