CH391L/S13/In vitro Selection of FNAs: Difference between revisions
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(New page: Category:CH391L_S13 ==Introduction== ==Types of Functional Nucleic Acids== <!--Regardless of the length of the eventual product, synthetic DNA constructs are built from some co...) |
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Revision as of 15:08, 8 February 2013
Introduction
Types of Functional Nucleic Acids
Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.
Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
